Human PDGF-BB Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DBB00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (10 µL), Platelet-poor EDTA Plasma (100 µL), Platelet-poor Heparin Plasma (100 µL)
Sensitivity
15 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human PDGF-BB Quantikine ELISA Kit
The Quantikine Human PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PDGF-BB in cell culture supernates, serum, or plasma. It contains E. coli-expressed recombinant human PDGF-BB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-BB.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human PDGF-BB
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 268 | 639 | 935 | 251 | 655 | 918 |
| Standard Deviation | 11.3 | 28.4 | 26.8 | 22.4 | 38.5 | 59.3 |
| CV% | 4.2 | 4.4 | 2.9 | 8.9 | 5.9 | 6.5 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 310 | 759 | 1093 | 287 | 772 | 1082 |
| Standard Deviation | 14.0 | 18.6 | 25.5 | 21.9 | 56.9 | 89.8 |
| CV% | 4.5 | 2.4 | 2.3 | 7.6 | 7.4 | 8.3 |
Recovery for Human PDGF-BB Quantikine ELISA Kit
The recovery of PDGF-BB spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 94 | 86-102 |
| Platelet-poor EDTA Plasma (n=6) | 101 | 88-115 |
| Platelet-poor Heparin Plasma (n=6) | 103 | 89-112 |
| Serum (n=6) | 102 | 93-114 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of PDGF-BB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human PDGF-BB Quantikine ELISA Kit
Human PDGF-BB ELISA Cell Culture Supernate Standard Curve
Human PDGF-BB ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF-BB
PDGF-BB (Platelet-Derived Growth Factor BB) is a homodimeric molecule expressed by hepatocytes and nonresorbing osteoclasts, and it promotes osteoblast development and bone formation. It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization. The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta. PDGF-BB has been shown to bind the alpha/alpha homodimer, alpha/beta heterodimer, and the beta/beta homodimer in vitro, and act through the beta/beta homodimer in vivo.
Long Name
Platelet-derived Growth Factor BB
Alternate Names
PDGFBB
Gene Symbol
PDGFB
Additional PDGF-BB Products
Product Documents for Human PDGF-BB Quantikine ELISA Kit
Product Specific Notices for Human PDGF-BB Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.