Human P-Selectin/CD62P ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DPSE00
Key Product Details
Assay Length
1.25 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
Sensitivity
0.121 ng/mL
Assay Range
0.781-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human P-Selectin/CD62P Quantikine ELISA Kit
The Quantikine® Human P-Selectin/CD62P Immunoassay is a 1.25 hour solid-phase ELISA designed to measure human P-Selectin in cell culture supernates, serum, and plasma. It contains recombinant human P-Selectin and antibodies raised against the recombinant factor. Results obtained using natural human P-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human P-Selectin.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Rrecombinant and natural human P-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human P-Selectin in this assay. No medical histories were available for the donors used in
this study.
ND=Non-detectable
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Serum (n=33) | 82.6 | 43.5-119 | 20.0 |
| EDTA plasma (n=33) | 35.3 | 18.3-57.4 | 8.78 |
| Heparin plasma (n=33) | 33.7 | 18.6-50.6 | 7.40 |
| Sample Type | Mean of Detectable (ng/mL) | % Detectable | Range (ng/mL) |
| Citrate plasma (n=33) | 25.8 | 97 | ND-37.9 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 6.54 | 19.3 | 25.6 | 6.51 | 19.9 | 30.5 |
| Standard Deviation | 0.442 | 1.47 | 2.29 | 0.484 | 1.57 | 3.64 |
| CV% | 6.8 | 7.6 | 8.9 | 7.4 | 7.9 | 11.9 |
Recovery for Human P-Selectin/CD62P Quantikine ELISA Kit
The recovery of Human P-Selectin spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 88-107 |
| Citrate Plasma (n=4) | 92 | 81-111 |
| EDTA Plasma (n=4) | 92 | 77-103 |
| Heparin Plasma (n=4) | 95 | 78-112 |
| Serum (n=4) | 93 | 77-104 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human P-Selectin were diluted with sample diluent to produce samples with values within the dynamic range of the assay. <br>
Scientific Data Images for Human P-Selectin/CD62P Quantikine ELISA Kit
Human P-Selectin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: P-Selectin/CD62P
P-Selectin, also known as CD62P, GMP-140, PADGEM, and LECAM-3, is a transmembrane adhesion protein that is upregulated on activated platelets and vascular endothelial cells. It binds to hematopoietic cell PSGL-1/CD162 with highly clustered O-linked sialyl Lewis-x (sLex ) tetrasaccharides and sulfated tyrosine, as well as to heparin sulfate, Versican, and CD42b/GPIb alpha. P-Selectin mediates the initial interaction of circulating leukocytes with activated endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. It is therefore critical for leukocyte extravasation at sites of inflammation and leukocyte involvement in thrombus formation at sites of vascular injury.
Alternate Names
CD62P, GMP140, GRMP, PADGEM, PSEL, SELP
Gene Symbol
SELP
Additional P-Selectin/CD62P Products
Product Documents for Human P-Selectin/CD62P Quantikine ELISA Kit
Product Specific Notices for Human P-Selectin/CD62P Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.