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Human Mesothelin Quantikine ELISA Kit

Catalog # DMSLN0 | R&D Systems, Inc. a Bio-Techne Brand
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DMSLN0

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (50 µL)

Sensitivity

0.022 ng/mL

Assay Range

0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)

Product Summary for Human Mesothelin Quantikine ELISA Kit

The Quantikine Human Mesothelin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Mesothelin in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human Mesothelin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Mesothelin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Mesothelin.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human Mesothelin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 0.83 2.56 5.54 0.91 2.80 5.94
Standard Deviation 0.034 0.082 0.193 0.057 0.130 0.360
CV% 4.0 3.2 3.5 6.2 4.6 6.1

Recovery for Human Mesothelin Quantikine ELISA Kit

The recovery of Mesothelin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 104 91-113
EDTA Plasma (n=4) 97 88-109
Heparin Plasma (n=4) 97 86-112
Saliva (n=4) 102 91-112
Serum (n=4) 99 92-114
Urine (n=4) 103 86-115

Linearity

To assess the linearity of the assay, samples containing high concentrations of Mesothelin were serially diluted Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Mesothelin ELISA Linearity

Scientific Data Images for Human Mesothelin Quantikine ELISA Kit

Human Mesothelin ELISA Standard Curve

Human Mesothelin ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Mesothelin

Mesothelin, also known as CAK1 and ERC, is derived from a precursor that also includes Megakaryocyte Potentiating Factor (MPF). Following cleavage of the precursor, Mesothelin remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

Product Documents for Human Mesothelin Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Mesothelin Quantikine ELISA Kit

For research use only

Citations for Human Mesothelin Quantikine ELISA Kit (3)

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  • Human Mesothelin Quantikine ELISA Kit
    Name: Adam Guess
    Sample Tested: Cell Culture Supernates
    Verified Customer | Posted 08/17/2018
    Human Mesothelin Quantikine ELISA Kit DMSLN0

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.