Human M-CSF Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DMC00B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Saliva (100 µL), Urine (100 µL)
Sensitivity
47.3 pg/mL
Assay Range
78.1-5000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human M-CSF Quantikine ELISA Kit
The Quantikine Human M-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human M-CSF in cell culture supernates, serum, plasma, saliva, and urine. It contains CHO cell-expressed recombinant human M-CSF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human M-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human M-CSF.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human M-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 248 | 751 | 1503 | 280 | 827 | 1620 |
| Standard Deviation | 19.4 | 16.1 | 49.0 | 30.2 | 76.0 | 87.7 |
| CV% | 7.8 | 2.1 | 3.3 | 10.7 | 9.2 | 5.4 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 357 | 951 | 1767 | 343 | 1019 | 1956 |
| Standard Deviation | 17.4 | 24.9 | 49.4 | 43.4 | 84.1 | 88.8 |
| CV% | 4.9 | 2.6 | 2.8 | 12.6 | 8.3 | 4.5 |
Recovery for Human M-CSF Quantikine ELISA Kit
The recovery of M-CSF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 90-107 |
| EDTA Plasma (n=4) | 100 | 93-106 |
| Heparin Plasma (n=4) | 99 | 93-106 |
| Serum (n=4) | 101 | 96-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of M-CSF were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human M-CSF Quantikine ELISA Kit
Human M-CSF ELISA Cell Culture Supernates/Saliva/Urine Standard Curve
Human M-CSF ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: M-CSF
M-CSF (CSF-1) is the primary regulator of macrophage survival, proliferation and differentiation. It is essential for macrophage-related functions such as bone resorption, vascular development, and innate immunity. It signals through M-CSF R on monocytes, tissue macrophages, and osteoblasts. Both M-CSF and its receptor can be expressed by tumor cells, allowing M-CSF to act as an autocrine growth factor for cancer cells. Full length M-CSF is expressed as a transmembrane dimer, but various additional forms can be generated by alternative splicing, differential glycosylation, chondroitin sulfate proteoglycan modification, and proteolytic shedding.
Long Name
Macrophage Colony Stimulating Factor
Alternate Names
CSF-1, CSF1, Lanimostim, MCSF
Gene Symbol
CSF1
Additional M-CSF Products
Product Documents for Human M-CSF Quantikine ELISA Kit
Product Specific Notices for Human M-CSF Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.