Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY6030-05
Discontinued Product
DY6030-05 has been discontinued.
An alternative/replacement product is available:
DY6030B-05. View all Indoleamine 2,3-dioxygenase/IDO products.
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
0.469-30 ng/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Indoleamine 2,3-dioxygenase (IDO). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
Human Indoleamine 2,3-dioxygenase / IDO ELISA Standard Curve
Kit Contents for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Indoleamine 2,3-dioxygenase/IDO
Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine. IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN gamma, IFN alpha, IFN beta, and IL-10. IDO has an antimicrobial function and induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia.
Alternate Names
3dioxygenase, IDO, IDO1, INDO, Indoleamine 2
Gene Symbol
IDO1
Additional Indoleamine 2,3-dioxygenase/IDO Products
Product Documents for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
Product Specific Notices for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
For research use only
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