Human IL-13 R alpha 2 DuoSet ELISA

Name: Human IL-13 R alpha 2 DuoSet ELISA
Brand: R&D Systems
SKU: DY614

Catalog # DY614 | R&D Systems, Inc. a Bio-Techne Brand

Citations (5)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	0.312 - 20 ng/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human IL-13 R alpha 2 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Technical Data
Citations (5) / Reviews

Summary for Human IL-13 R alpha 2 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-13 Ralpha2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human IL-13 R alpha 2 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human IL-13 R alpha 2 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human IL-13 R alpha 2 DuoSet ELISA

	Human IL-13 R alpha 2 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-13 R alpha 2

Two members of the type 5 subfamily of type I cytokine receptors can serve as receptors for IL-13. IL-13 can bind to IL-13 R alpha 1 (CD213a1; previously designated IL-13 R alpha or NR4) with low affinity, then recruits the IL-4 R alpha chain to form a high affinity receptor, causing downstream STAT6 activation. Alternately, IL-13 can bind IL-13 R alpha 2 (CD213a2) with high affinity; this interaction does not cause activation of STAT6, but does induce TGF-beta production. IL-13 R alpha 1 and IL-13 R alpha 2 each have three extracellular fibronectin type III domains, two cytokine receptor homology modules and a WSXWS motif typical of the class I cytokine receptor family, but IL-13 R alpha 2 has a much shorter cytoplasmic tail. IL-13 R subunits can be expressed on monocytes, macrophages, fibroblasts, human B cells, basophils, eosinophils, endothelial cells, and smooth muscle cells.

Item	Value
Long Name	Interleukin 13 Receptor alpha 2
Entrez Gene IDs	3598 (Human); 16165 (Mouse)
Alternate Names	CD213a2, CD213a2 antigen, CT19, IL-13 R alpha 2, IL-13 receptor subunit alpha-2, IL-13R, IL-13R subunit alpha-2, IL-13R-alpha-2, IL-13Ra2, IL13BP, IL13R, IL13R alpha 2, IL13RA2, Interleukin-13-binding protein, cancer/testis antigen 19, interleukin 13 binding protein, interleukin 13 receptor alpha 2 chain, interleukin 13 receptor, alpha 2, interleukin-13 receptor subunit alpha-2

Citations for Human IL-13 R alpha 2 DuoSet ELISA (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-13 R alpha 2 DuoSet ELISA include:

Species: Human
Sample Types: Serum

D Keresztes et al. (2021-12-30), Comparative proteome analysis identified CD44 as a possible serum marker for docetaxel resistance in castration-resistant prostate cancer Journal of Cellular and Molecular Medicine, 2021-10(0).
PMID: 34970839
Species: Human
Sample Types: BALF

O'Toole M et al. (2008-02-25), A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects. Clin. Exp. Allergy, 2008-038(4):594-601.
PMID: 18307523
Species: Human
Sample Types: Cell Culture Supernates

Matsumura M et al. (2007-06-19), Endogenous metalloprotease solubilizes IL-13 receptor alpha2 in airway epithelial cells. Biochem. Biophys. Res. Commun., 2007-0360(2):464-9.
PMID: 17603012
Species: Human
Sample Types: BALF

Daines MO et al. (2006-11-30), Allergen-dependent solubilization of IL-13 receptor alpha2 reveals a novel mechanism to regulate allergy. J. Allergy Clin. Immunol., 2006-1119(2):375-83.
PMID: 17140645
Species: Human
Sample Types: Cell Lysates

Daines MO et al. (2006-06-15), Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling. J. Immunol., 2006-0176(12):7495-501.
PMID: 16751396

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