Human IL-12/IL-23 p40 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DP400
Key Product Details
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)
Sensitivity
15 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human IL-12/IL-23 p40 Quantikine ELISA Kit
The Quantikine Human IL-12 p40 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-12 p40 in cell culture supernates, serum, and plasma. It contains recombinant human IL-12 p40 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-12 p40. Results obtained using natural human IL-12 p40 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-12 p40.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-12 p40
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 119 | 290 | 871 | 127 | 315 | 891 |
| Standard Deviation | 5.1 | 11.4 | 35.5 | 11.8 | 19.8 | 60.6 |
| CV% | 4.3 | 3.9 | 4.1 | 9.3 | 6.3 | 6.8 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 151 | 380 | 1138 | 160 | 399 | 1145 |
| Standard Deviation | 9.9 | 27.2 | 62.3 | 11.0 | 22.3 | 63.0 |
| CV% | 6.6 | 7.2 | 5.5 | 6.9 | 5.6 | 5.5 |
Recovery for Human IL-12/IL-23 p40 Quantikine ELISA Kit
The recovery of IL-12 p40 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 96 | 85-105 |
| Citrate Plasma (n=5) | 100 | 85-112 |
| EDTA Plasma (n=5) | 101 | 92-113 |
| Heparin Plasma (n=5) | 96 | 89-106 |
| Serum (n=5) | 98 | 86-112 |
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-12 p40 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-12/IL-23 p40 Quantikine ELISA Kit
Human IL-12/IL-23 p40 Homodimer ELISA Cell Culture Supernate Standard Curve
Human IL-12/IL-23 p40 Homodimer ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-12/IL-23 p40
IL-12 (Interleukin-12) is a secreted heterodimeric cytokine that contains disulfide-linked p35 and p40 subunits. The p40 subunit is also a subunit of the heterodimeric IL-23. IL-12 is produced by macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, microglia, and non-germinal center B cells. It acts through a receptor complex that contains the ligand binding IL-12 R alpha 1 and the signal transducing IL-12 R alpha 2. IL-12 can enhance cytotoxic activity and induce Interferon-gamma production in NK cells, T cells and dendritic epidermal T cells. In conjunction with IL-23 and IL-27, IL-12 promotes the development of a Th1 immune response.
Long Name
Interleukin 12/Interleukin 23 p40
Alternate Names
CLMF2, IL-12B, IL12 p40, IL12B, NKSF2
Entrez Gene IDs
Gene Symbol
IL12B
Additional IL-12/IL-23 p40 Products
Product Documents for Human IL-12/IL-23 p40 Quantikine ELISA Kit
Product Specific Notices for Human IL-12/IL-23 p40 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.