Human IL-10 QuicKit ELISA

Name: Human IL-10 QuicKit ELISA
Brand: R&D Systems
SKU: QK1064

R&D Systems, part of Bio-Techne | Catalog # QK1064

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Product Datasheet / COA / SDS

Key Product Details

Assay Length:

80 minutes

Human IL-10 QuicKit ELISA Summary:

The Quantikine QuicKit Human IL-10 Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human IL-10 levels in cell culture supernates, serum, and plasma.It contains recombinant human IL-10 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human IL-10 showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-10.

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Technical Data
Citations / Reviews

Product Specifications for Human IL-10 QuicKit ELISA

Measurement	Quantitative ELISA
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human IL-10.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Recovery

The recovery of IL-10 is spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	96	87-105
EDTA Plasma (n=2)	84	75-90
Heparin Plasma (n=2)	78	65-97
Serum (n=2)	92	80-105

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-10 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human IL-10 QuicKit ELISA

	Human IL-10 Quantikine QuicKit ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-10

Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.

Item	Value
Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 100034187 (Equine); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names	CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL-10, IL-10MGC126451, IL10, IL10A, TGIF, interleukin 10, interleukin-10

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Human IL-10 QuicKit ELISA

R&D Systems, part of Bio-Techne | Catalog # QK1064

Key Product Details

View all IL-10 ELISA Kits »

Product Specifications for Human IL-10 QuicKit ELISA

Recovery

Linearity

Scientific Data Examples for Human IL-10 QuicKit ELISA

Preparation and Storage

Background: IL-10

Customer Reviews for Human IL-10 QuicKit ELISA

Product Documents for Human IL-10 QuicKit ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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