Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DLA50
Key Product Details
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL), Citrate Plasma (200 µL)
Sensitivity
1 pg/mL
Assay Range
3.9-250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
The Quantikine Human IL-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 alpha in cell culture supernates, serum, and plasma. It contains recombinant human IL-1 alpha and antibodies raised against recombinant human IL-1 alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 alpha showed linear curves that were parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1 alpha.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Sample Values
Serum/Plasma - Forty samples from apparently healthy volunteers were evaluated for the
presence of human IL-1 alpha, and all had levels which fell below the lowest IL-1 alpha standard,
3.9 pg/mL. No medical histories were available for the donors used in this study.
Cell Culture Supernates - Human peripheral blood mononuclear cells (5 x 106
cells/mL) were
cultured in RPMI supplemented with 5% fetal bovine serum, 50 μM beta-mercaptoethanol,
2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were
cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates
were removed on days 1 and 5 and assayed for levels of human IL-1 alpha.
| Condition | Day 1 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | 85 | 23 |
| Stimulated | 1330 | 395 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 26.9 | 122 | 191 | 20.5 | 60.0 | 121 |
| Standard Deviation | 0.4 | 4.6 | 6.6 | 1.7 | 3.1 | 5.2 |
| CV% | 1.5 | 3.8 | 3.5 | 8.3 | 5.2 | 4.3 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 28.6 | 125 | 185 | 27.9 | 123 | 187 |
| Standard Deviation | 0.4 | 1.4 | 4.0 | 1.2 | 4.7 | 6.3 |
| CV% | 1.4 | 1.1 | 2.2 | 4.3 | 3.8 | 3.4 |
Recovery for Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
The recovery of IL-1 alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 91-104 |
| Citrate Plasma (n=6) | 97 | 71-119 |
| EDTA Plasma (n=6) | 104 | 84-121 |
| Heparin Plasma (n=6) | 104 | 72-126 |
| Serum (n=6) | 107 | 74-127 |
Linearity
To assess the linearity of the assay, the following biological samples spiked with high concentrations of IL-1 alpha were diluted with the appropriate Calibrator Diluent and then assayed.
Scientific Data Images for Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
Human IL-1 alpha/IL-1F1 ELISA Calibrator Diluent RD5-5 Standard Curve
Human IL-1 alpha/IL-1F1 ELISA Calibrator Diluent RD6C Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 alpha/IL-1F1
IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signalling.
Long Name
Interleukin 1 alpha
Alternate Names
BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM
Entrez Gene IDs
Gene Symbol
IL1A
Additional IL-1 alpha/IL-1F1 Products
Product Documents for Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
Product Specific Notices for Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.