Human IFN-gamma Quantikine QuicKit ELISA Best Seller

Name: Human IFN-gamma Quantikine QuicKit ELISA
Brand: R&D Systems
SKU: QK285
Rating: 4 (1 reviews)

Catalog # QK285 | R&D Systems, Inc. a Bio-Techne Brand

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Citations (1)

Product Datasheet / COA / SDS

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Key Product Details

Assay Length:	80 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity:	2.56 pg/mL
Assay Range:	31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Human IFN-gamma Quantikine QuicKit ELISA Summary:	The Quantikine QuicKit Human IFN-gamma Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IFN-gamma levels in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-gamma and antibodies raised against the recombinant protein. Results obtained using natural human IFN-gamma showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.

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Technical Data
Citations (1) / Reviews (1)

Product Specifications for Human IFN-gamma Quantikine QuicKit ELISA

Measurement	Quantitative ELISA
Conjugate	HRP
Reactivity	Human
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	20	20	10	10
Mean (pg/mL)	198	1156	211	1233
Standard Deviation	3.76	20.2	20.4	56.9
CV%	1.9	1.8	9.6	4.6

Recovery

The recovery of human IFN-gamma spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	92	83-102
EDTA Plasma (n=4)	107	88-132
Serum (n=4)	93	74-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IFN-gamma were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Scientific Data Examples for Human IFN-gamma Quantikine QuicKit ELISA

	Human IFN-gamma QuicKit ELISA Spiked Recovery Competitor Comparison IFN-gamma is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Serum recovery is 115% compared to 144% for the top competitor. Plasma recovery is 120% compared to 135% for the top competitor. Cell Culture Supernates recovery is 118% compared to 79% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.
	Human IFN-gamma QuicKit ELISA Spiked Linearity Competitor Comparison IFN-gamma is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-126% compared to 71%-85% for the top competitor.
	Human IFN-gamma Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Item	Value
Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names	IFG, IFI, IFN-gamma, IFNG, IFNgamma, Immune interferon, interferon gamma, interferon, gamma

Citations for Human IFN-gamma Quantikine QuicKit ELISA (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human IFN-gamma Quantikine QuicKit ELISA include:

Species: Mouse
Sample Types: BALF

Y Song et al. (2023-02-02), Gene expression profiles and bioinformatics analysis in lung samples from ovalbumin-induced asthmatic mice BMC pulmonary medicine, 2023-023(1):50.
PMID: 36726128

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Human IFN-gamma Quantikine QuicKit ELISA

Name: Anonymous

Sample Tested: Urine

Verified Customer | Posted 11/16/2022

Quick, easy to follow protocol. Urine tested at 2-fold dilution and did not observe a matrix effect.

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Product Documents for Human IFN-gamma Quantikine QuicKit ELISA

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COA

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