Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

Name: Human IFN-gamma Quantikine HS ELISA Kit
Brand: R&D Systems
SKU: HSDIF0
Rating: 5 (2 reviews)

A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

Human IFN-gamma Quantikine HS ELISA Kit Best Seller

Catalog # HSDIF0 | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (2)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Assay Length:	4.0 hours
Sample Type & Volume Required Per Well:	Serum (100 uL), EDTA Plasma ( Not Assigned), Heparin Plasma (100 uL)
Sensitivity:	0.173 pg/mL
Assay Range:	0.469 - 30 pg/mL (Serum, Plasma, EDTA Plasma, Heparin Plasma)
Human IFN-gamma Quantikine HS ELISA Kit Summary:	The Quantikine^® HS Human IFN-gamma Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IFN-gamma in serum and plasma. It contains HEK293-expressed recombinant human IFN-gamma and antibodies raised against the recombinant protein. Results obtained using natural human IFN-gamma showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.

View Full Technical Details for Human IFN-gamma Quantikine HS ELISA Kit

Product Specifications for Human IFN-gamma Quantikine HS ELISA Kit

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human IFN-gamma.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Serum, Plasma, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	1.83	6.1	12.8	2.15	6.33	12.4
Standard Deviation	0.084	0.187	0.253	0.21	0.421	0.898
CV%	4.6	3.1	2	9.8	6.7	7.2

Recovery

The recovery of human IFN-gamma spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	99	90-108
Heparin Plasma (n=4)	99	92-109
Serum (n=4)	97	85-109

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human IFN-gamma were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human IFN-gamma Quantikine HS ELISA Kit

	Human IFN-gamma HS Quantikine ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Item	Value
Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names	IFG, IFI, IFN-gamma, IFNG, IFNgamma, Immune interferon, interferon gamma, interferon, gamma

Citations for Human IFN-gamma Quantikine HS ELISA Kit (2)

Showing 1 - 2 of 2 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human IFN-gamma Quantikine HS ELISA Kit include:

Species: Human
Sample Types: Serum

ST Kim et al. (2023-02-10), Interferon and interferon-induced cytokines as markers of impending clinical progression in ANA+ individuals without a systemic autoimmune rheumatic disease diagnosis Arthritis Research Therapy, 2023-02-10;25(1):21.
PMID: 36765391
Species: Human
Sample Types: Serum

N Todorovi?- et al. (2022-02-23), The prognostic significance of serum interferon-gamma (IFN-gamma) in hormonally dependent breast cancer Cytokine, 2022-0152(0):155836.
PMID: 35219004

There are no citations that match your criteria.

Showing 1 - 2 of 2 citations Showing All

Customer Reviews for Human IFN-gamma Quantikine HS ELISA Kit (2)

5 out of 5

2 customer ratings

5 stars

100%

4 stars

3 stars

2 stars

1 star

Showing 1 - 2 of 2 reviews Showing All

Filter By:

Human IFN-gamma Quantikine HS ELISA Kit

Name: Anonymous

Sample Tested: Serum-free Cell Culture Supernates

Verified Customer | Posted 12/03/2021
Human IFN-gamma Quantikine HS ELISA Kit

Name: Anonymous

Sample Tested: Serum

Verified Customer | Posted 10/21/2021

There are no reviews that match your criteria.

Showing 1 - 2 of 2 reviews Showing All

FAQs for Human IFN-gamma Quantikine HS ELISA Kit

Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

Human IFN-gamma Quantikine HS ELISA Kit Best Seller

Catalog # HSDIF0 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Product Specifications for Human IFN-gamma Quantikine HS ELISA Kit

Precision

Recovery

Linearity

Scientific Data Examples for Human IFN-gamma Quantikine HS ELISA Kit

Preparation and Storage

Background: IFN-gamma

Citations for Human IFN-gamma Quantikine HS ELISA Kit (2)

Customer Reviews for Human IFN-gamma Quantikine HS ELISA Kit (2)

Human IFN-gamma Quantikine HS ELISA Kit

Human IFN-gamma Quantikine HS ELISA Kit

FAQs for Human IFN-gamma Quantikine HS ELISA Kit

Product Documents for Human IFN-gamma Quantikine HS ELISA Kit

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne 品牌

保持关注