Which isoform(s) does R&D Systems Human Glypican 3 Quantikine ELISA kit (Catalog DGLY30) detect?

Name: Human Glypican 3 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DGLY30
Rating: 5 (1 reviews)

The Human Glypican 3 Quantikine ELISA Kit is designed to detect Human Glypican 3 isoform 1, and we have confirmed this in-house. It is possible that the assay also detects other Human Glypican 3 isoforms, however, R&D Systems has not performed this evaluation.

Human Glypican 3 Quantikine ELISA Kit

Catalog # DGLY30 | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (1)

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (100 uL), Cell Lysates (20 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity:	20.6 pg/mL
Assay Range:	78.1 - 5,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Human Glypican 3 Quantikine ELISA Kit Summary:	The Quantikine Human Glypican 3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Glypican 3 levels in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human Glypican 3 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Glypican 3 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human Glypican 3 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human Glypican 3.

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Technical Data
Citations (1) / Reviews (1) / FAQs

Product Specifications for Human Glypican 3 Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human Glypican 3.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	412	1208	2387	466	1231	2359
Standard Deviation	12	48	79.6	38.4	75.3	143
CV%	2.9	4	3.3	8.2	6.1	6.1

Recovery

The recovery of human Glypican 3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	99	88-112
EDTA Plasma (n=4)	88	81-95
Heparin Plasma (n=4)	89	81-96
Serum (n=4)	89	82-97

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Glypican 3 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human Glypican 3 Quantikine ELISA Kit

	Human Glypican 3 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Glypican 3

The glypicans (GPC) constitute a family of glycosylphosphatidylinositol (GPI)-anchored, heparan sulfate proteoglycans. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site.

Mutations in Glypican 3 cause a rare disorder in humans, Simpson-Golabi-Behmel Syndrome, which is characterized by pre- and postnatal overgrowth of multiple tissues and organs and an increased risk for developing embryonic tumors. These features are also present in the mouse knock-out of Glypican 3 indicating that Glypican 3 regulates cell survival and inhibits cell proliferation during development. Glypican 3 has been implicated in regulating many different signaling pathways including: IGF, FGF, BMP and Wnt. An endoproteolytic processing of Glypican 3 by proprotein convertases is required for the modulation of Wnt signaling. Direct interaction of Glypican 3 with FGF-basic has been observed and is mediated by the heparan sulfate chains.

Item	Value
Entrez Gene IDs	2719 (Human); 14734 (Mouse); 25236 (Rat); 102137748 (Cynomolgus Monkey)
Alternate Names	DGSX, GPC3, GTR2-2, Glypican 3, Intestinal protein OCI-5, MXR7, OCI-5, OCI5, SGB, SGBS, SGBS1SDYS, glypican proteoglycan 3, glypican-3, heparan sulphate proteoglycan, secreted glypican-3

Citations for Human Glypican 3 Quantikine ELISA Kit (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human Glypican 3 Quantikine ELISA Kit include:

Species: Human
Sample Types: Serum

C Xie et al. (2017-08-29), Development of an Affimer-antibody combined immunological diagnosis kit for glypican-3 Sci Rep, 2017-07(1):9608.
PMID: 28852111

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Customer Reviews for Human Glypican 3 Quantikine ELISA Kit (1)

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Human Glypican 3 Quantikine ELISA Kit

Name: Anonymous

Sample Tested: HEK cell supernatant,mouse serum

Verified Customer | Posted 07/24/2020

Transfect the hGPC3 on HEK cells and collect the supernatant and measured. Crystal Clear.

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FAQs for Human Glypican 3 Quantikine ELISA Kit

Which isoform(s) does R&D Systems Human Glypican 3 Quantikine ELISA kit (Catalog DGLY30) detect?

The Human Glypican 3 Quantikine ELISA Kit is designed to detect Human Glypican 3 isoform 1, and we have confirmed this in-house. It is possible that the assay also detects other Human Glypican 3 isoforms, however, R&D Systems has not performed this evaluation.

Product Documents for Human Glypican 3 Quantikine ELISA Kit

Product Datasheets

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COA

SDS

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Human Glypican 3 Quantikine ELISA Kit

Catalog # DGLY30 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Human Glypican 3 Quantikine ELISA Kit

Precision

Recovery

Linearity

Scientific Data Examples for Human Glypican 3 Quantikine ELISA Kit

Preparation and Storage

Background: Glypican 3

Citations for Human Glypican 3 Quantikine ELISA Kit (1)

Customer Reviews for Human Glypican 3 Quantikine ELISA Kit (1)

Human Glypican 3 Quantikine ELISA Kit

FAQs for Human Glypican 3 Quantikine ELISA Kit

Product Documents for Human Glypican 3 Quantikine ELISA Kit

Product Datasheets

COA

Certificate of Analysis

SDS

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