Human Galectin-3 ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DGAL30
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
0.085 ng/mL
Assay Range
0.313-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human Galectin-3 Quantikine ELISA Kit
The Quantikine Human Galectin-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Galectin-3 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Galectin-3 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Galectin-3 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Galectin-3.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Galectin-3. This assay cross-reacts 58% with Mouse Galectin-3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 0.78 | 2.27 | 5.06 | 0.81 | 2.51 | 5.29 |
| Standard Deviation | 0.03 | 0.10 | 0.15 | 0.07 | 0.20 | 0.36 |
| CV% | 3.8 | 4.4 | 3.0 | 8.6 | 8.0 | 6.8 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 0.79 | 2.46 | 5.11 | 0.80 | 2.59 | 5.50 |
| Standard Deviation | 0.03 | 0.09 | 0.18 | 0.05 | 0.16 | 0.32 |
| CV% | 3.8 | 3.7 | 3.5 | 6.3 | 6.2 | 5.8 |
Recovery for Human Galectin-3 Quantikine ELISA Kit
The recovery of Galectin-3 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 96-106 |
| EDTA Plasma (n=4) | 105 | 93-113 |
| Heparin Plasma (n=4) | 103 | 95-110 |
| Serum (n=4) | 104 | 94-115 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of human Galectin-3 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Galectin-3 Quantikine ELISA Kit
Human Galectin-3 ELISA/LGALS3 ELISA Cell Culture Supernate Standard Curve
Human Galectin-3 ELISA/LGALS3 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Galectin-3
Galectin-3, also known as Mac-2, L29, CBP35, and etaBP, is a secreted lectin that acts in anti-microbial immunity by pathogen opsonization, macrophage recruitment, and the activation of mast cells and neutrophils. It can also contribute to chronic inflammation and fibrosis. It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis.
Alternate Names
AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2
Gene Symbol
LGALS3
Additional Galectin-3 Products
Product Documents for Human Galectin-3 Quantikine ELISA Kit
Product Specific Notices for Human Galectin-3 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.