Human Erythropoietin Quantikine IVD ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DEP00B
Human Erythropoietin Quantikine IVD ELISA Kit
Key Product Details
Assay Length
4.5 hours (Benchtop), 2.5 hours (Shaker)
Sample Type & Volume Required Per Well
Serum (100 µL), EDTA Plasma (100 µL)
Sensitivity
0.6 mU/mL
Assay Range
2.5-200 mU/mL (Serum, EDTA Plasma)
Product Summary for Human Erythropoietin Quantikine IVD ELISA Kit
The Quantikine IVD Human Epo ELISA uses a monoclonal antibody and polyclonal antibody conjugate in a sandwich ELISA format to provide a method that is quicker, more sensitive and more specific than the bioassay. The assay is designed to measure Epo levels in serum or plasma
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Epo.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with other substances.
Sample Values
Erythropoietin concentrations were obtained from 123 normal individuals from the Minneapolis/St. Paul, Minnesota area. Using the nonparametric method for the analysis of reference values outlined in the NCCLS publication "How to Define, Determine, and Utilize Reference Intervals in the Clinical Laboratory" (NCCLS Document C28-P; Vol. 12, No. 2) the following reference ranges (2.5-97.5 percentile) were established for Epo in serum and EDTA plasma. Each testing laboratory should establish its own normal range.
Patients suffering from polycythemia rubra vera may have Epo concentrations within the normal range, whereas those suffering from secondary polycythemia may have elevated concentrations of serum Epo (1). Polycythemia rubra vera patients who undergo phlebotomy may have elevated serum Epo concentrations.
Epo Normal Ranges
| Serum | EDTA plasma |
| 3.3-16.6 mlU/mL | 3.1-14.9 mlU/mL |
Patients suffering from polycythemia rubra vera may have Epo concentrations within the normal range, whereas those suffering from secondary polycythemia may have elevated concentrations of serum Epo (1). Polycythemia rubra vera patients who undergo phlebotomy may have elevated serum Epo concentrations.
Patients suffering from most anemias will present with higher than normal concentrations of serum Epo, whereas those suffering from anemia associated with chronic renal failure may have serum Epo concentrations within the normal range of this assay (2). Anemic patients who receive transfusions may exhibit lower than expected serum Epo concentrations.
Abnormally high concentrations of serum Epo may also be observed in various other pathological states including renal neoplasms, benign tumors, polycystic kidney disease, renal cysts and hydronephrosis (3).
The results of this assay should be used in conjunction with information available from clinical evaluations and other diagnostic procedures.
Precision
Intra-Assay Precision (Precision within an assay) Assay precision was assessed as outlined in the CLSI publication CLSI EP05-A3, Evaluation of
Precision of Quantitative Measurement Procedures; Third Edition. The following modifications
were made to the basic design described in the CLSI guideline. Three reagents lots were used
instead of one. For within laboratory precision, 10 assays were run by 5 operators for each
reagent lot over a period of at least 20 days with no more than 2 assays run per day.
For within assay precision, 20 replicates of each control were run for each reagent lot. Three
control pools were run in both the benchtop and shaker protocols of the Quantikine™ IVD
Human Epo ELISA for both within laboratory and within assay precision.
The results of each assay were tabulated and the within assay precision and within laboratory
precision of each control pool was calculated.
Benchtop Protocol
Shaker Protocol
Within Laboratory Precision
Within Assay Precision
Within Laboratory Precision
Within Assay Precision
Control
1
2
3
1
2
3
1
2
3
1
2
3
Mean (mIU/mL)
9.42
40.1
116
10.6
41.3
115
9.79
40.9
116
8.81
39.4
114
n=
30
30
30
20
20
20
30
30
30
20
20
20
%CV
11.0
7.2
5.4
4.7
4.9
3.4
12.0
5.9
5.2
4.1
2.5
3.2
Inter-Assay Precision (Precision between assays)
Recovery for Human Erythropoietin Quantikine IVD ELISA Kit
Recovery was estimated by adding recombinant human Epo at three different levels across the range of the assay into six plasma and six serum specimens. Mean percent recoveries are shown in the table below:
| Benchtop Protocol | Shaker Protocol | |||
| Specimen Type | Average % Recovery | Range | Average % Recovery | Range |
| Serum (n=6) | 100 | 84-115 | 101 | 96-107 |
| EDTA Plasma (n=6) | 99 | 81-114 | 96 | 84-105 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Epo were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.<div><br></div><div><table border="1" cellspacing="0" cellpadding="0" width="642" style="border-collapse:collapse;border:none;"> <tbody><tr> <td width="208" colspan="2" rowspan="2" style="width:155.8pt;border:solid #BCBDC0 1.0pt;background:#E7E6E6;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";color:black;"> </b><b><span style="font-size:10.0pt; font-family:"Arial",sans-serif;Times New Roman";"></b> </td> <td width="217" colspan="2" valign="top" style="width:162.9pt;border:solid #BCBDC0 1.0pt; border-left:none; background:#E7E6E6;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";color:black;">Benchtop Protocol</b><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";"></b> </td> <td width="217" colspan="2" valign="top" style="width:162.9pt;border:solid #BCBDC0 1.0pt; border-left:none; background:#E7E6E6;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";color:black;">Shaker Protocol</b><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";"></b> </td> </tr> <tr> <td width="109" valign="top" style="width:81.5pt;border-top:none;border-left: none;border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;background:#E7E6E6;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";color:black;">Serum</b><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";"></b> </td> <td width="109" valign="top" style="width:81.4pt;border:solid #BCBDC0 1.0pt; border-left:none; background:#E7E6E6;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";color:black;">EDTA Plasma</b><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";"></b> </td> <td width="109" valign="top" style="width:81.5pt;border-top:none;border-left: none;border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;background:#E7E6E6;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";color:black;">Serum</b><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";"></b> </td> <td width="109" valign="top" style="width:81.4pt;border-top:none;border-left: none;border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;background:#E7E6E6;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";color:black;">EDTA Plasma</b><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";"></b> </td> </tr> <tr> <td width="45" rowspan="2" style="width:33.95pt;border:solid #BCBDC0 1.0pt; border-top:none; padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">1:2 </td> <td width="162" style="width:121.85pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">Average % of Expected </td> <td width="109" style="width:81.5pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">111 </td> <td width="109" style="width:81.4pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">110 </td> <td width="109" style="width:81.5pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">105 </td> <td width="109" style="width:81.4pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">109 </td> </tr> <tr> <td width="162" style="width:121.85pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">Range (%) </td> <td width="109" style="width:81.5pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">106-118 </td> <td width="109" style="width:81.4pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">104-118 </td> <td width="109" style="width:81.5pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">99-109 </td> <td width="109" style="width:81.4pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">101-117 </td> </tr> <tr> <td width="45" rowspan="2" style="width:33.95pt;border:solid #BCBDC0 1.0pt; border-top:none; padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">1:4 </td> <td width="162" style="width:121.85pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">Average % of Expected </td> <td width="109" style="width:81.5pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">109 </td> <td width="109" style="width:81.4pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">106 </td> <td width="109" style="width:81.5pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">102 </td> <td width="109" style="width:81.4pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">105 </td> </tr> <tr> <td width="162" style="width:121.85pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">Range (%) </td> <td width="109" style="width:81.5pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">100-123 </td> <td width="109" style="width:81.4pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">94-122 </td> <td width="109" style="width:81.5pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">94-113 </td> <td width="109" style="width:81.4pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">95-119 </td> </tr> <tr style="height:1.0pt"> <td width="45" rowspan="2" style="width:33.95pt;border:solid #BCBDC0 1.0pt; border-top:none; padding:7.5pt 7.5pt 7.5pt 7.5pt;height:1.0pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">1:8 </td> <td width="162" style="width:121.85pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt; height:1.0pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">Average % of Expected </td> <td width="109" style="width:81.5pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt; height:1.0pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">105 </td> <td width="109" style="width:81.4pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt; height:1.0pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">99 </td> <td width="109" style="width:81.5pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt; height:1.0pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">96 </td> <td width="109" style="width:81.4pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt; height:1.0pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">98 </td> </tr> <tr> <td width="162" style="width:121.85pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">Range (%) </td> <td width="109" style="width:81.5pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">93-123 </td> <td width="109" style="width:81.4pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">92-118 </td> <td width="109" style="width:81.5pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">89-106 </td> <td width="109" style="width:81.4pt;border-top:none;border-left:none; border-bottom:solid #BCBDC0 1.0pt;border-right:solid #BCBDC0 1.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"> <span style="font-size:10.0pt;font-family:"Arial",sans-serif;Times New Roman";">89-114 </td> </tr> </tbody></table><br></div>
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Erythropoietin/EPO
Erythropoietin/EPO is a renal glycoprotein hormone that promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha. Erythropoietin/EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis.
R&D System products include Erythropoietin antibodies, proteins, and ELISAs. Our products cover multiple species and our EPO antibodies are validated for multiple applications including Neutralization, Western blot, ELISA, and Immunohistochemistry.
Alternate Names
ECYT5, EPO, MVCD2
Gene Symbol
EPO
Additional Erythropoietin/EPO Products
Product Documents for Human Erythropoietin Quantikine IVD ELISA Kit
Product Specific Notices for Human Erythropoietin Quantikine IVD ELISA Kit
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