Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DCX130
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (25 µL)
Sensitivity
3.97 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Product Summary for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit
The Quantikine Human BLC/BCA-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BLC/BCA-1 in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant human BLC/BCA-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BLC/BCA-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring BLC/BCA-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human BLC/BCA-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Saliva - Samples from apparently healthy volunteers were evaluated for the
presence of human BLC/BCA-1 in this assay. No medical histories were available for the donors
used in this study.
*Twenty-five additional serum samples were tested and values of 10.6 pg/mL, 379 pg/mL, and 638 pg/mL were observed in three
samples. The remaining samples were consistent with the range listed above.
ND=Non-Detectable
ND=Non-detectable
| Sample Type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum* (n=35) | 81.9 | 39.4-252 | 43.5 |
| EDTA plasma (n=35) | 62.5 | 29.4-246 | 42.7 |
| Heparin plasma (n=35) | 63.5 | 21.7-240 | 44.1 |
| Sample Type | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Saliva (n=10) | 67.4 | 70 | ND-284 |
Cell Culture Supernates:
Human peripheral blood cells (1 x 106
cells/mL) were cultured in RPMI supplemented with
10% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin,
and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with
10 μg/mL PHA. Aliquots of the cell culture supernate were removed and assayed for levels of
human BLC/BCA-1.
| Condition | Day 1 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | ND | ND |
| Stimulated | 11 | 640 |
THP-1 human acute monocytic leukemia cells were cultured in RPMI supplemented with
10% fetal bovine serum, stimulated with 1.0 μg/mL of recombinant human IFN-gamma for
eight hours, and then stimulated with 1.0 μg/mL of LPS overnight. An aliquot was removed,
assayed for detectable human BLC/BCA-1, and measured 15,922 pg/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 50.2 | 136 | 294 | 54.0 | 138 | 285 |
| Standard Deviation | 2.2 | 4.5 | 8.0 | 5.1 | 13.2 | 24.8 |
| CV% | 4.3 | 3.3 | 2.7 | 9.4 | 9.6 | 8.7 |
Recovery for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit
The recovery of natural BLC/BCA-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 87-110 |
| EDTA Plasma (n=4) | 99 | 92-107 |
| Heparin Plasma (n=4) | 101 | 92-115 |
| Serum (n=4) | 99 | 86-108 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of natural BLC/BCA-1 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit
Human CXCL13/BLC/BCA-1 ELISA Standard Curve
Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit
Correlation between European League Against Rheumatism Sjögren’s Syndrome Disease Activity Index (ESSDAI) scores and concentrations of nine screened serum proteins analyzed by ELISA in the validation cohort of patients with primary Sjögren’s syndrome (n = 58). The nine proteins were CXCL13, TNF receptor 2 (TNF-R2), CD48, B-cell activating factor (BAFF), programmed cell death protein 1 ligand 2 (PD-L2), lymphocyte activation gene-3 (LAG-3), beta2-microglobulin ( beta2MG), ephrin type-B receptor 2 (EPHB2), and CD163. Pearson’s correlation test was used. The correlation coefficients (r) and P values are shown in the table. P <0.05 was considered significant Image collected and cropped by CiteAb from the following open publication (https://arthritis-research.biomedcentral.com/articles/10.1186/s13075-016-1006-1), licensed under a CC-BY license. Not internally tested by R&D Systems.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL13/BLC/BCA-1
CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naive B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer's patch development.
Alternate Names
ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13
Gene Symbol
CXCL13
Additional CXCL13/BLC/BCA-1 Products
Product Documents for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit
Product Specific Notices for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.