Human CCL26/Eotaxin-3 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCC260B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
0.215 pg/mL
Assay Range
3.13-200 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, Plasma)
Product Summary for Human CCL26/Eotaxin-3 Quantikine ELISA Kit
The Quantikine® Human CCL26/Eotaxin-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Eotaxin-3 levels in cell culture supernates, cell lysates, serum, and plasma. It contains E.
coli-expressed, recombinant human Eotaxin-3 and antibodies raised against the recombinant factor. Results obtained for naturally occurring human Eotaxin-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Eotaxin-3.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Reactivity
Human
Specificity
Natural and recombinant human Eotaxin-3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 43.6 | 86.0 | 131 | 44.4 | 91.7 | 139 |
| Standard Deviation | 0.570 | 1.06 | 1.77 | 3.66 | 7.79 | 11.6 |
| CV% | 1.3 | 1.2 | 1.4 | 8.2 | 8.5 | 8.3 |
Recovery for Human CCL26/Eotaxin-3 Quantikine ELISA Kit
The recovery of human Eotaxin-3 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 102 | 92-119 |
| Cell Lysis Buffer (n=1) | 97 | 94-99 |
| EDTA Plasma (n=5) | 94 | 84-105 |
| Heparin Plasma (n=5) | 95 | 86-109 |
| Serum (n=5) | 93 | 81-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Eotaxin-3 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CCL26/Eotaxin-3 Quantikine ELISA Kit
Human Eotaxin-3 ELISA Standard Curve
Human Eotaxin-3 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL26/Eotaxin-3
CCL26, also known as Eotaxin-3 and SCYA26, is a small cytokine that is a member of the CC chemokine family. It is only 94 amino acids (aa) in length, with aa 1-23 serving as a putative signal peptide. Human CCL26/Eotaxin-3 has a predicted molecular weight of approximately 11 kDa, and the mature protein only shares 27% aa sequence identity with the mouse ortholog. CCL26/Eotaxin-3 is constitutively expressed at low levels in various tissues including in the heart and ovary. Its expression in upregulated in vascular endothelial cells by IL-13 and IL-4. CCL26/Eotaxin-3 is a selective agonist for CCR3, as serves as a chemoattractant for cells that express CCR3, such as eosinophils, basophils, and Th2 cells. It has also been shown to act as an antagonist for the receptors CCR1 and CCR5. CCL26/Eotaxin-3 plays a role in allergic reactions.
Additional CCL26/Eotaxin-3 Products
Product Documents for Human CCL26/Eotaxin-3 Quantikine ELISA Kit
Product Specific Notices for Human CCL26/Eotaxin-3 Quantikine ELISA Kit
For research use only
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