Human/Canine/Porcine Insulin ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DINS00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
2.15 pmol/L
Assay Range
15.6-500 pmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human/Canine/Porcine Insulin Quantikine ELISA Kit
The Quantikine Human/Canine/Porcine Insulin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Insulin in cell culture supernates, serum, and plasma. It contains S. cerevisiae-expressed recombinant human Insulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Insulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Insulin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human, Porcine, Canine
Specificity
Recombinant human Insulin and natural human, canine, and porcine Insulin. This assay cross-reacts 0.25% with Human Proinsulin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy, fasting volunteers were evaluated for the
presence of human/canine/porcine Insulin in this assay. No medical histories were available for the
donors used in this study.
ND=Non-detectable
ND=Non-detectable
| Human Samples | Mean (pmol/L) | Range (pmol/L) | Standard Deviation (pmol/L) |
| Serum (n=34) | 58.0 | 17.2-218 | 41 |
| EDTA plasma (n=34) | 60.3 | 15.8-208 | 39 |
| Heparin plasma (n=34) | 59.4 | 16.4-190 | 45 |
| Canine Samples | Mean (pmol/L) | Range (pmol/L) | Standard Deviation (pmol/L) |
| Serum (n=10) | 58.8 | 16.7-190 | 52 |
| Heparin plasma (n=5) | 78.3 | 21.5-146 | 56 |
| Canine Samples | Mean of Detectable (pmol/L) | % Detectable | Range (pmol/L) |
| EDTA plasma (n=5) | 96.4 | 80 | ND-145 |
| Porcine Samples | Mean of Detectable (pmol/L) | % Detectable | Range (pmol/L) |
| Serum (n=10) | 22.6 | 90 | ND-34.3 |
| EDTA plasma (n=5) | 27.1 | 80 | ND-40.8 |
| Heparin plasma (n=5) | 30.4 | 80 | ND-40.9 |
Cell Culture Supernates - Human peripheral blood mononuclear cells (PBL) were cultured in DMEM
supplemented with 5% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL
penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with
10 μg/mL PHA for 1 and 5 days. Aliquots of the cell culture supernates were removed and assayed
for human/canine/porcine Insulin. No detectable levels were observed.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pmol/L) | 66.8 | 158 | 299 | 62.2 | 148 | 288 |
| Standard Deviation | 2.58 | 5.89 | 11.9 | 4.60 | 10.2 | 21.5 |
| CV% | 3.9 | 3.7 | 4.0 | 7.4 | 6.9 | 7.5 |
Recovery for Human/Canine/Porcine Insulin Quantikine ELISA Kit
The recovery of Insulin spiked to levels throughout the range of the assay in various matrices was evaluated
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 85-108 |
| EDTA Plasma (n=4) | 95 | 86-102 |
| Heparin Plasma (n=4) | 92 | 85-105 |
| Serum (n=4) | 96 | 87-103 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Insulin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human/Canine/Porcine Insulin Quantikine ELISA Kit
Human/Porcine/Canine Insulin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Insulin
Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. Insulin processes out part of the G, leaving two parallel, but disulfide linked segments; the IGFs only process at the ends, retaining the basic configuration.
Alternate Names
IDDM2, ILPR, INS, IRDN, MODY10
Gene Symbol
INS
Additional Insulin Products
Product Documents for Human/Canine/Porcine Insulin Quantikine ELISA Kit
Product Specific Notices for Human/Canine/Porcine Insulin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.