Human BMP-4 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DBP400
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Bone Extracts (50 µL)
Sensitivity
3.68 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Bone Extracts)
Product Summary for Human BMP-4 Quantikine ELISA Kit
The Quantikine Human BMP-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure BMP-4 levels in bone tissue extracts and cell culture supernates. It contains NS0-expressed recombinant human BMP-4 and antibodies raised against the recombinant factor.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human BMP-4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Bone Extracts, Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 301 | 719 | 1351 | 289 | 734 | 1429 |
| Standard Deviation | 15.9 | 22.5 | 60.1 | 16.9 | 42.7 | 75.4 |
| CV% | 5.3 | 3.1 | 4.4 | 5.8 | 5.8 | 5.3 |
Recovery for Human BMP-4 Quantikine ELISA Kit
The recovery of recombinant human BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| 2 M Guanidine-HCI (n=2) | 96 | 93-103 |
| 2 M Urea (n=2) | 97 | 91-105 |
| Cell Culture Media (n=8) | 100 | 91-109 |
Linearity
The following samples were spiked with rhBMP-4 and then diluted with Calibrator Diluent. The samples were then serially diluted and tested to assess linearity of the assay.
Scientific Data Images for Human BMP-4 Quantikine ELISA Kit
Human BMP-4 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BMP-4
BMP-4 forms homodimers or heterodimers with BMP-2 and signals through receptor complexes composed of type I (primarily ActRIA or BMPR-IA) and type II (primarily ActRIIA or BMPR-II) receptors. BMP-4 plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.
Long Name
Bone Morphogenetic Protein 4
Alternate Names
BMP2B, BMP2B1, BMP4, MCOPS6, OFC11
Gene Symbol
BMP4
Additional BMP-4 Products
Product Documents for Human BMP-4 Quantikine ELISA Kit
Product Specific Notices for Human BMP-4 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.