Human B7-1/CD80 DuoSet ELISA

Name: Human B7-1/CD80 DuoSet ELISA
Brand: R&D Systems
SKU: DY140
Rating: 5 (1 reviews)

Catalog # DY140 | R&D Systems, Inc. a Bio-Techne Brand

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human B7-1/CD80 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Technical Data
Citations / Reviews (1)

Summary for Human B7-1/CD80 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human B7-1/CD80. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human B7-1/CD80 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human B7-1/CD80 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human B7-1/CD80 DuoSet ELISA

	Human B7-1 / CD80 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: B7-1/CD80

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20 - 100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells.

Item	Value
Entrez Gene IDs	941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names	Activation B7-1 antigen, B7, B7-1, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 molecule, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80, costimulatory factor CD80, costimulatory molecule variant IgV-CD80

Citations for Human B7-1/CD80 DuoSet ELISA

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Customer Reviews for Human B7-1/CD80 DuoSet ELISA (1)

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Human B7-1/CD80 DuoSet ELISA

Name: Anonymous

Sample Tested: THP-1 human acute monocytic leukemia cell line

Verified Customer | Posted 05/02/2017

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Product Documents for Human B7-1/CD80 DuoSet ELISA

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Human B7-1/CD80 DuoSet ELISA

Catalog # DY140 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary for Human B7-1/CD80 DuoSet ELISA

Product Specifications for Human B7-1/CD80 DuoSet ELISA

Kit Contents for Human B7-1/CD80 DuoSet ELISA

Other Reagents Required

Scientific Data Examples for Human B7-1/CD80 DuoSet ELISA

Preparation and Storage

Background: B7-1/CD80

Citations for Human B7-1/CD80 DuoSet ELISA

Customer Reviews for Human B7-1/CD80 DuoSet ELISA (1)

Customer Images

Human B7-1/CD80 DuoSet ELISA

Product Documents for Human B7-1/CD80 DuoSet ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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