Human HSP27 [p Ser15] ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62151

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Key Product Details

Sample Type & Volume Required Per Well

Cell lysates, plasma, and serum (100 uL)

Sensitivity

10.15 pg/ml

Assay Range

31.25 - 1000 pg/ml

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

Crossreactivity: Phopho HSP27 (Ser15) (100%), HSP27 (non-phospho) (0.885%), HSP70 (0.023%) and <0.016%: HSP25, HSP40, HSP90, alphaB Crystallin, alphaA Crystallin

Precision

Intra-Assay Precision (Precision within an assay) Low Concentration %CV - 14.2% Medium Concentration %CV - 9.3% High Concentration %CV - 4.0%

Inter-Assay Precision (Precision between assays) Low Concentration %CV - 9.9% Medium Concentration %CV - 9.6% High Concentration %CV - 7.1%

Recovery for Human HSP27 [p Ser15] ELISA Kit (Colorimetric)

Recovery

HeLa Cells (human) - 92%
Liver Microsomes - 103%
Serum (human) - 99%
EDTA Plasma - 103-109%

Scientific Data Images for Human HSP27 [p Ser15] ELISA Kit (Colorimetric)

ELISA: Human HSP27 [p Ser15] ELISA Kit (Colorimetric) [NBP2-62151]

ELISA: Human HSP27 [p Ser15] ELISA Kit (Colorimetric) [NBP2-62151]

ELISA: Human HSP27 [p Ser15] ELISA Kit (Colorimetric) [NBP2-62151] - A typical standard curve for HSP27 [p Ser15] ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human HSP27 [p Ser15] ELISA Kit (Colorimetric)

  • 5X Extraction reagent
  • Antibody
  • Assay buffer 27
  • Conjugate
  • Microtiter plate
  • Standard
  • Stop solution 2
  • TMB Substrate
  • Wash buffer concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

Additional HSP27 Products

Product Documents for Human HSP27 [p Ser15] ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human HSP27 [p Ser15] ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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