Human, Mouse HSF1 [p Ser326] ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62150

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NBP2-62150
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Key Product Details

Sample Type & Volume Required Per Well

Cell lysates and tissue (100 uL)

Sensitivity

61 pg/ml

Assay Range

0.39 - 12.5 ng/ml

View all HSF1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human, Mouse

Precision

Intra-Assay Precision (Precision within an assay) Low Concentration %CV - 1.1% Medium Concentration %CV - 1.0% High Concentration %CV - 2.4%

Inter-Assay Precision (Precision between assays) Low Concentration %CV - 2.6% Medium Concentration %CV - 7.1% High Concentration %CV - 2.9%

Recovery for Human, Mouse HSF1 [p Ser326] ELISA Kit (Colorimetric)

Recovery

3T3 - 100%
Heat-shocked 3T3 - 107% HeLa - 100%
Heat-shocked HeLa - 105%

Scientific Data Images for Human, Mouse HSF1 [p Ser326] ELISA Kit (Colorimetric)

ELISA: Human, Mouse HSF1 [p Ser326] ELISA Kit (Colorimetric) [NBP2-62150]

ELISA: Human, Mouse HSF1 [p Ser326] ELISA Kit (Colorimetric) [NBP2-62150]

ELISA: Human, Mouse HSF1 [p Ser326] ELISA Kit (Colorimetric) [NBP2-62150] - A typical standard curve for HSF1 [p Ser326] ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human, Mouse HSF1 [p Ser326] ELISA Kit (Colorimetric)

  • Antibody
  • Assay buffer
  • Conjugate
  • Extraction reagent #1
  • Microtiter plate
  • Standard
  • Stop solution
  • TMB substrate
  • Wash buffer concentrate.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HSF1

Human cells respond to heat stress by inducing the binding of a pre-existing transcriptional activator (heat shock factor, HSF) to DNA (1). Induction of heat shock protein (HSP) gene expression by stress is initiated by binding of HSF1 to HSP gene promoters to increase their transcription. The cytoprotective functions of these HSPs are essential for cell survival, and thus it is critical that inducible HSP gene expression be executed rapidly and efficiently. There is an interaction between heat shock factor 1 (HSF1) and symplekin, a protein known to form a complex with the polyadenylation factors CstF and CPSF. HSF1-symplekin complexes are detected only after stress treatment, and these two proteins co-localize in punctate nuclear structures in stressed cells (2). A chaperone/Hsp functioning as repressor of heat shock transcription factor (HSF) could make activation of hsp genes dependent on protein unfolding. It has been concluded that Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1 (3).

Long Name

Heat Shock Transcription Factor 1

Alternate Names

HSTF1

Gene Symbol

HSF1

Additional HSF1 Products

Product Documents for Human, Mouse HSF1 [p Ser326] ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human, Mouse HSF1 [p Ser326] ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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