Creatinine Parameter Assay Kit Best Seller

Name: Creatinine Parameter Assay Kit
Brand: R&D Systems
SKU: KGE005
Rating: 5 (5 reviews)

R&D Systems, part of Bio-Techne | Catalog # KGE005

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

< 1 hour

Sample Type & Volume Required Per Well

Urine (10 µL)

Sensitivity

0.07 mg/dL

Assay Range

0.31-20 mg/dL (Urine)

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Product Summary for Creatinine Parameter Assay Kit

The Parameter Creatinine assay is a 30 minute chemical analysis designed to measure Creatinine in urine.

Product Specifications

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

Biotin

Reactivity

Multi-Species

Specificity

Measures Creatinine levels in urine

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.

Interference

Interference observed with other substances.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	1.67	5.28	9.75	1.55	4.92	9.25
Standard Deviation	0.053	0.168	0.339	0.085	0.259	0.366
CV%	3.2	3.2	3.5	5.5	5.3	4.0

Recovery for Creatinine Parameter Assay Kit

The recovery of Creatinine spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Urine (n=4)	96	92-97

Linearity

To assess the linearity, nine urine samples were serially diluted with deionized or distilled water and assayed in two lots of kit reagents.

Scientific Data Images for Creatinine Parameter Assay Kit

Multi-species Creatinine ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage conditions vary by component. See product insert for storage instructions.

Background: Creatinine

Creatinine is a non-enzymatic metabolite of phospho-Creatine, a molecule used primarily by muscle tissue as a store for high-energy phosphate that can be utilized for the production of ATP. Creatinine diffuses into the blood and is excreted by the kidneys at a relatively constant rate. Creatinine levels in the urine are used as a measurement of renal function.