Canine IFN-gamma Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # CAIF00
Key Product Details
Assay Length
4 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
60 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Canine IFN-gamma Quantikine ELISA Kit
The Quantikine Canine IFN-gamma Immunoassay is a 4 hour solid-phase ELISA designed to measure canine IFN-gamma in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring canine IFN-gamma showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine IFN-gamma.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Reactivity
Canine
Specificity
Natural and recombinant canine IFN-gamma
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 111 | 459 | 1234 | 119 | 457 | 1315 |
| Standard Deviation | 10.1 | 35.1 | 92.4 | 10.1 | 21.5 | 64.8 |
| CV% | 9.1 | 7.6 | 7.5 | 8.5 | 4.7 | 4.9 |
Recovery for Canine IFN-gamma Quantikine ELISA Kit
The recovery of canine IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 103 | 92-111 |
| EDTA Plasma (n=4) | 96 | 86-102 |
| Heparin Plasma (n=4) | 98 | 93-106 |
| Serum (n=4) | 96 | 83-107 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of canine IFN-gamma in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Canine IFN-gamma Quantikine ELISA Kit
Canine IFN-gamma ELISA Standard Curve
Detection of Canine IFN-gamma by ELISA
Serum IFN-gamma induction was associated with NHS-IL12 dose and the observation of adverse events.IFN-gamma levels were measured using ELISA techniques. Induction of IFN-gamma was detectable in dogs treated with NHS-IL12 at a dose of 1.6 mg/m2 or higher. IFN-gamma levels spiked sharply at 24 hours post-treatment and returned to undetectable levels by 48 hours. Elevated serum IFN-gamma (> 100 pg/ml) was associated with increased risk for toxicity. However the highest level of IFN-gamma induction was not directly linked to the most severe (Grade 4 or 5) adverse events. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26091536), licensed under a CC-BY license. Not internally tested by R&D Systems.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-gamma
IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
Long Name
Interferon gamma
Alternate Names
IFG, IFI, IFNG, IFNgamma
Entrez Gene IDs
Gene Symbol
IFNG
Additional IFN-gamma Products
Product Documents for Canine IFN-gamma Quantikine ELISA Kit
Product Specific Notices for Canine IFN-gamma Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.