When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?

Name: MagCellect Plus Human CD45+ Cell Isolation Kit
Brand: R&D Systems
SKU: MAGH125

It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

MagCellect Plus Human CD45+ Cell Isolation Kit

R&D Systems, part of Bio-Techne | Catalog # MAGH125

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Product Datasheet / COA / SDS

Discontinued Product

MAGH125 has been discontinued. View all CD45 products.

Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD45

CD45 is a transmembrane protein tyrosine phosphatase. It is expressed at high levels on the cell surface of all nucleated hematopoietic cells and their precursors. Alternative splicing of CD45 mRNA results in the expression of several distinct isoforms that are differentially expressed in cells of different lineages and activation states.

Long Name

Cluster of Differentiation 45

Alternate Names

CD45, LCA, PTPRC, T200 Glycoprotein

Entrez Gene IDs

5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)

Gene Symbol

PTPRC

Additional CD45 Products

Product Documents for MagCellect Plus Human CD45+ Cell Isolation Kit

Product Datasheets

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COA

SDS

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Product Specific Notices for MagCellect Plus Human CD45+ Cell Isolation Kit

For research use only

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