MagCellect Human CD4+ T Cell Isolation Kit
R&D Systems, part of Bio-Techne | Catalog # MAGH102
Key Product Details
Species
Human
Product Summary for MagCellect Human CD4+ T Cell Isolation Kit
Kit Summary
Why Isolate Regulatory T cells?
CD4+ T cells develop in the thymus and differentiate into various subsets of more specialized T lymphocytes. The development of these subsets is induced by distinct signals and is controlled by distinct transcription factors. CD4+ T cell subsets produce characteristic combinations of cytokines which enables them to exert diverse functions. CD4+ T cells are critically involved in recruiting and activating other immune cells, dampening ongoing immune responses, and maintaining immunologic memory. The relative expansion of CD4+ subsets changes dramatically in response to local environmental stresses or disease status. The isolation of CD4+ T cells is an important step in the preparation of multiple subsets including Th1, Th2, and Th17 cells. See Details
Prominent Characteristics of CD4+ T Cell Subsets
Th1 Cells
- Protect against intracellular infections
- Contribute to disease pathogenesis in autoimmunity
- Develop in response to IL-12 and type 1 Interferons
- Require transcription factors STAT4 and T-bet for differentiation and function
- Secrete IFN-gamma, TNF-alpha, IL-2
Th2 Cells
- Protect against intestinal helminths and extracellular bacteria
- Support B cell-dependent humoral immune responses
- Contribute to the development of allergic inflammation
- Develop in response to IL-4 and IL-2
- Require transcription factors STAT6 and GATA3 for differentiation and function
- Secrete IL-4, IL-5, IL-9, IL-13, IL-17E/IL-25
Th17 Cells
- Protect against extracellular bacteria and fungi
- Oppose some functions of regulatory T cells
- Mediate autoimmune and inflammatory disease pathogenesis
- Develop in response to TGF-beta and IL-6
- Expand and survive in the presence of IL-21 and IL-23
- Require transcription factor RORgammaT
- Secrete TNF-alpha, IL-6, IL-9, IL-17A, IL-17F, IL-21, IL-22, IL-26
Regulatory T cells (Treg)
- Limit the development and progression of immune responses
- Oppose some functions of Th17 cells
- Suppress the development of autoimmunity
- Develop in response to TGF-beta and IL-2
- Require transcription factor FoxP3 for differentiation and function
- Secrete TGF-beta, IL-9, IL-10, IL-35
Memory T cells (either CD4+ or CD8+)
- Survive for extended time after antigen is cleared
- Provide rapid immune activation in response to subsequent infection
- Differentiate into multiple subsets
- Reside in characteristic tissues depending on expressed profile of chemokine receptors
- Secrete IFN-gamma upon activation as well as other subset-specific cytokines
Other Subsets
Follicular helper T cells (Tfh) provide support for germinal center development and B cell responses. Their differentiation is under the control of the transcription factor Bcl6. Th9 cells and Th22 cells are characterized by the secretion of IL-9 and IL-22, respectively.
References:
Farber, D.L. et al. (2014) Nat. Rev. Immunol. 14:24.Magombedze, G. et al. (2013) Front. Physiol. 4:206.
Reagents Provided
The MagCellect Human CD4+ T Cell Isolation Kit contains the following reagents that allow for isolation of highly pure human CD4+ cells.
- MagCellect Human CD4+ T Cell Biotinylated Antibody Cocktail; 1 mL in PBS containing BSA
- MagCellect Streptavidin Ferrofluid; 1.25 mL in PBS containing BSA and preservatives
- MagCellect Plus Buffer (10X) – 10 mL of 10X concentrated buffer
*This kit contains sufficient reagents to process 1 x 109 total cells.
Stability and Storage
Store all reagents at 2 °C to 8 °C. DO NOT FREEZE.
Scientific Data Images for MagCellect Human CD4+ T Cell Isolation Kit
Enrichment of CD4+ T Cells from Human Peripheral Blood Mononuclear Cells Using the MagCellect CD4+ T Cell Isolation Kit.
Ficolled human PBMC before (A) and after (B) isolation of CD4+ T cells were stained with PE-conjugated Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # FAB100P) and FITC-conjugated Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # FAB3791F).Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD4
CD4 is a transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th9, Th17,Th22, Tfh, and Treg cells which regulate humoral and cellular immunity. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. CD4 binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1.
Alternate Names
CD4
Entrez Gene IDs
Gene Symbol
CD4
Additional CD4 Products
Product Documents for MagCellect Human CD4+ T Cell Isolation Kit
Product Specific Notices for MagCellect Human CD4+ T Cell Isolation Kit
For research use only
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