For applications utilizing embryonic stem (ES) cells, the success is highly dependent on identifying a suitable lot of Fetal Bovine Serum. ES Cell Qualified Fetal Bovine Serum is pre-screened for exceptional performance with embryonic stem cells, minimizing the need for evaluating serum lots.
Key Benefits:
Utilizes our highest quality FBS
Extended testing on human embryonic stem (hES) cells
Screened for colony morphology, pluripotency, and plating efficiency
Maintains hES cells in an undifferentiated state
Low endotoxin and hemoglobin levels
USDA-Approved FBS of Mexico/Central American origin
Manufactured in our ISO 9001:2015 certified facility
Only the highest quality Fetal Bovine Serum, also known as Fetal Calf Serum (FCS), lots are selected for further testing regarding suitability for culturing embryonic stem cells. This screening includes plating efficiency, colony morphology, and cytotoxicity assays. ES Qualified FBS lots must offer outstanding performance with embryonic stem cells while maintaining the cells in their valuable undifferentiated state. All lots of Embryonic Stem Cell Qualified FBS are manufactured in our ISO 9001:2015 certified facility.
Heat Inactivation - Serum is inactivated by raising the temperature to 56 °C for 30 minutes under controlled conditions. The objective of heat inactivation is to destroy complement activity in the serum without affecting the growth-promoting characteristics of the product. Removal of complement activity from the serum is not required for most cell cultures, but may be necessary for cultures that are sensitive to the complement activity. Since heat inactivation of the serum may, to some extent, decrease the growth performance properties of the serum, this procedure should only be performed if actually required for optimal cell growth. Researchers should evaluate the applicability of heat inactivation in regards to their own application. If heat inactivation is required, the process should be carefully controlled to avoid increased formation of crystalline and flocculent precipitates, gelling of serum proteins and excessive loss of growth performance. Significant damage to serum can occur when it is subjected to higher than required temperatures or heated over extended lengths of time.
Collection, Processing, and Testing of R&D Systems FBS
Collection and Processing - R&D Systems FBS is manufactured under a process that meets USDA requirements for animal products and ensures the product is traceable back to the date and location of collection. Our FBS manufacturing adheres to closed-system collection, processing in positive pressure, HEPA-filtered clean rooms, and triple 0.1 um filtration to remove contaminants. Strict control of the process from collection through processing enables us to provide a stable, consistent performing, and traceable supply of quality serum throughout global fluctuations in serum availability and regulatory requirements.
Biochemical Testing - R&D Systems FBS undergo biochemical profiling including total protein, albumin, globulin, IgG, total bilirubin, glucose, iron, cholesterol, triglycerides, sodium and potassium. View the product insert for a complete list of biochemical profiling components, which may vary between grades.
Chemical Analysis - Each lot of FBS is tested for osmolality, pH, hemoglobin, endotoxin (LAL method) and microbiological contamination (using methods recommended by the U.S. Pharmacopeia).
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