ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free Best Seller
R&D Systems, part of Bio-Techne | Catalog # CCM037
For potent and highly proliferative NK Cells.
Key Product Details
Features
Animal Component-Free Media for the ex vivo expansion and culture of human Natural Killer lymphocytes.
Key Benefits
- Supports feeder- and serum-free expansion of NK cells
- Phenol red-free formulation
- Animal component-free media to support translational research
- Supports NK cell expansion from PBMCs or purified CD3+-depleted PBMCs
- Protocol to support feeder-cell G-Rex culture process
- Protocol to support feeder-free G-Rex culture process
Species
Human
Product Summary for ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free
Why Use ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free?
Natural Killer (NK) cells play an important role in both the adaptive and innate immune responses that control infection, autoimmunity, and tumor immunosurveillance. Due to their intrinsic and non-specific anti-tumor activity, human NK cells have been employed as anti-cancer therapies. Robust expansion and maintenance of NK Cells is critical to facilitate NK cell therapy.
Versatility of ExCellerate Human NK Cell Expansion Media
ExCellerate Human NK Cell Expansion Media is optimized for NK cell expansion when used in combination with Recombinant Human Cytokines from R&D Systems; however, this versatile media also provides robust growth using a variety of cytokine combinations (IL-2, IL-15, IL-12, IL-18, and IL-21) and cell activation methods, including particle, bead-based, or plate-bound Human NKp46/NCR1 Antibody. The media supports both feeder-based and feeder-free expansion methods.
Scientific Data Images for ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free
Only ExCellerate CCM037 supports both feeder-free and serum-free expansion of NK cells.
Purified NK cells were plated at 2.5e6/cm2 in a 24-well G-Rex using media without serum supplementation. NK cells were stimulated with 250 IU/mL IL-2, 2 ng/mL IL-12 (at initial seeding only), and 10 ng/mL each of IL-15, IL-18, and IL-21. After 7 days, NK cells were reseeded at a density of 0.05e6/cm2 and grown for a total of 14 days. Data labels are cell viability at harvest.
ExCellerate CCM037 supports feeder-free expansion of NK cells better than competitor medias.
Purified NK cells were plated at 2.5e6/cm2 in a 24-well G-Rex using medias supplemented with 5% hAB serum. NK cells were stimulated with 250 IU/mL IL-2, 2 ng/mL IL-12 (at initial seeding only), and 10 ng/mL each of IL-15, IL-18, and IL-21. After 7 days, NK cells were reseeded at a density of 0.05e6/cm2 and grown for a total of 14 days. Data labels are cell viability at harvest.
NK cells grown in ExCellerate CCM037 with or without serum show similar phenotypes.
Day 14 expression profiles of NK cells grown in feeder-free, cytokine stimulated culture conditions either with or without serum supplementation, 3 donor average.
Kit Contents for ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free
1 L of ExCellerate Human NK Cell Expansion Media, Animal Component-Free
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the recommended temperature. Protect from light.
Storage
Store the unopened product at -20 °C. Do not use past expiration date. Protect from Light.
Product Specific Notices for ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free
FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
- The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
- This reagent should not be used beyond the expiration date indicated on the CoA.
- Results may vary due to variations among cells derived from different donors.
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