Cultrex Stem Cell Qualified RGF Basement Membrane Extract (BME) has been shown to provide an effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in culture.
Cultrex Stem Cell Qualified RGF Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex Stem Cell Qualified RGF BME has been shown to provide an effective surface for feeder-free stem cell attachment and maintenance of undifferentiated human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). It is useful for promoting the expansion of pluripotent stem cells or for the study of stem cell differentiation.
Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.
Product Specifications
Species
Mouse
Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
8-12 mg/mL as determined by Lowry assay
Endotoxin level
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing
Promotes the human induced pluripotent stem cell attachment.
Effectively maintains human iPSCs in a pluripotent state in a feeder-free stem cell culture.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Scientific Data Examples
Cultrex BME Improves Hematopoietic Stem Cell Differentiation from Induced Pluripotent Stem Cells (iPSCs).
Human iPSCs were grown for a minimum of 2 passages on either Cultrex Stem Cell Qualified RGF BME (Catalog # 3434-005-02) or a leading competitor's matrix, prior to creating embryoid bodies for hematopoietic stem cell differentiation. Cells pre-cultured on Cultrex Stem Cell Qualified RGF BME, showed a greater efficiency to develop into hematopoietic stem cells (CD34+, CD45+). Data courtesy of the Verneris Laboratory at the University of Colorado.
Colonies of iPSCs Grown on Cultrex Stem Cell Qualified RGF BME.
Human erythroblasts, reprogrammed into induced pluripotent stem cells (iPSCs), were cultured on plates coated with Cultrex Stem Cell Qualified RGF BME (Catalog # 3434-005-02). Example colonies are shown at 4X magnification. Data courtesy of the Harvard Stem Cell Core.
Preparation & Storage
Stability
Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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