Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME) is an extracellular matrix hydrogel that has been used extensively in general cell culture applications to study cell attachment, proliferation, and invasion. It is ideal for cultures that require limited reduced growth factor conditions.
Cultrex Reduced Growth Factor Basement Membrane Extract (Catalog # 3433-010-01) is equivalent to Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Catalog # A1413201 and A1413202).
Key Benefits:
Ideal for general cell culture application
Reduced growth factor formulation provides a more defined culture system
Polymerizes at 37°C to form a reconstituted basement membrane
Summary for Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear
Why Use Cultrex RGF BME?
Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. It is processed to reduce matrix-associated growth factors to provide a matrix that can be used to create a more defined model system. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex RGF BME can be used in multiple applications, including maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle, stem cells, and organoid/3-D cell cultures. It can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion, and in vivo tumorigenicity assays.
Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Scientific Data Examples for Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear
Assessment of Pluripotency on Cultrex RGF BME.
Alkaline Phosphatase (AP) staining was used to characterize the pluripotency of human induced pluripotent stem (iPS) cells cultured on Cultrex Reduced Growth Factor BME (Catalog # 3433-010-01). Images are shown in 4X and 10X magnification. Data courtesy of GABAeron.
Preparation & Storage
Stability
Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
