Purified stromal extracellular matrix protein that can promte cell adhesion for cell and tissue cultures
Key Product Details
Features:
Cultrex Rat Collagen I, Low Viscosity is a purified stromal extracellular matrix protein that is formulated as an easy to work with hydrogel for coating tissue culture vessels. It may also be used to supplement customized coatings, hydrogels, or medium formulations for cell culture.
Key Benefits:
Qualified for general cell culture applications
Lower in viscosity for easier handling
May be used as a hydrogel or thin coating for tissue culture vessels
Material provided at a standard concentration of ~3 mg/mL
Summary for Cultrex Rat Collagen I, Lower Viscosity
Why Use Cultrex Rat Collagen I, Lower Viscosity?
Cultrex Rat Collagen I, Lower Viscosity is purified from rat tail tendons and is provided at a standard concentration that is lower in viscosity, making it easier to work with. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37°C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 3 mg/mL.
Product Specifications for Cultrex Rat Collagen I, Lower Viscosity
Species
Rat
Source
Rat tail tendons
Protein Concentration
3 mg/mL
Endotoxin level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing
Supports cell attachment and spreading of HT1080 human fibrosarcoma cells.
Viral Testing
No mycoplasma contamination detected by PCR.
Preparation & Storage
Stability
Product is stable for a minimum of 2 months if stored at 2-8 °C
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at 2 - 8 °C. Do not freeze.
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