Can Cultrex Mouse Laminin I be used on glass cover slips?

Yes. Coating glass cover slips with Cultrex Laminin I is quite common and has been published extensively.

What is the recommended working concentration for Cultrex Laminin I?

The recommended working concentration for thin coating is 0.05-10 µg/cm2 . However, conditions must be optimized for each cell line or model. Cultrex Laminin I should be used at 6 mg/mL for 3-D culture applications.

Cultrex Mouse Laminin I, Pathclear Best Seller

Name: Cultrex Mouse Laminin I, Pathclear
Brand: R&D Systems
SKU: 3400-010-02
Rating: 5 (2 reviews)

R&D Systems, part of Bio-Techne | Catalog # 3400-010-02

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Product Datasheet / COA / SDS

Mouse Laminin I purified and qualified for use in cell culture.

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Product Summary
Product Specifications
Preparation & Storage
Background
Product Documents

Key Product Details

Features

Cultrex Mouse Laminin I, Pathclear is a purified basement membrane protein that has been developed and qualified as a extracellular matrix coating solution for cell attachment to tissue culture vessels.

Key Benefits

Produced and qualified for use in cell culture
Pathclear-tested to ensure a pathogen and virus-free material
Source tissue is LDEV-free

Species

Mouse

Product Summary for Cultrex Mouse Laminin I, Pathclear

Why Use Cultrex Mouse Laminin I?

Cultrex Mouse Laminin I, Pathclear is purified from Engelbreth-Holm-Swarm (EHS) tumor and is Pathclear-certified to be free of common pathogens. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation.

Negative for the presence of bacteria and fungi.

Product Specifications

Source

Murine Engelbreth-Holm-Swarm (EHS) tumor

Protein Concentration

1 mg/mL

Endotoxin Level

≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay

Sterility Testing

No bacterial or fungal growth detected following 14 days in culture

Cell Culture Testing

Supports cell attachment and spreading of MG63 human osteosarcoma cells.

Viral Testing

Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.

Formulation, Preparation, and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.

Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Stability

Product is stable for a minimum of 3 months from date of shipment. See lot specific Certificate of Analysis for expiration date.

Background: Laminin-1

Laminins are heterotrimeric, noncollagenous glycoproteins composed of combinations of five known alpha, four beta and three gamma chains in mammals. One alpha, beta and gamma subunit self-assembles to form at least 15 coiled-coil heterotrimers. Through specific interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes and survival. Some subunits, such as Laminin gamma1 (Laminin B2), are present in several laminins; Laminin-1 (EHS laminin), Laminin-2 (merosin), Laminin-3 (S-laminin), Laminin-4 (S-merosin), Laminin-6 (K-laminin) and Laminin-7 (KS-laminin). Others, such as the beta3 and gamma2 subunits, occur in only one known combination (e.g. with alpha3 in Laminin-5 / Epiligrin).