Cultrex Basement Membrane Extract, Type 2, Pathclear Best Seller
Catalog # 3532-010-02 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Features: | Cultrex Basement Membrane Extract (BME), Type 2 is specifically designed to support the establishment and expansion of robust organoid cultures. It's composition mimics the in vivo microenvironment to improve take rate and growth of organoid progenitor cells. |
Key Benefits: |
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Citations for Cultrex Basement Membrane Extract, Type 2, Pathclear (15)
Citations are publications that use Bio-Techne products. Selected citations for Cultrex Basement Membrane Extract, Type 2, Pathclear include:
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Species: Transgenic Mouse
Sample Types: Organoid
Applications: BioassayEG Foerster et al. (2023-02-14), ATG16L1 protects from interferon-gamma-induced cell death in the small intestinal crypt Mucosal Immunology, 2023-00(0).
PMID: 36792009 -
Species: Human
Sample Types: Organoid
Applications: BioassayS Shi et al. (2023-01-04), Modeling bile duct ischemia and reoxygenation injury in�human cholangiocyte organoids for screening of novel cholangio-protective agents EBioMedicine, 2023-088(0):104431.
PMID: 36608526 -
Species: Human
Sample Types: Organoid
Applications: BioassayM Oro? et al. (2022-09-27), The molecular network of the proteasome machinery inhibition response is orchestrated by HSP70, revealing vulnerabilities in cancer cells Cell Reports, 2022-040(13):111428.
PMID: 36170818 -
Species: Mouse, Transgenic Mouse
Sample Types: Organoid
Applications: BioassayH Daghero et al. (2022-08-24), Jejunum-derived NF-kappaB reporter organoids as 3D models for the study of TNF-alpha-induced inflammation Scientific Reports, 2022-012(1):14425.
PMID: 36002565 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayJ Di Domizio et al. (2022-01-19), The cGAS-STING pathway drives type I IFN immunopathology in COVID-19 Nature, 2022-00(0).
PMID: 35045565 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayMP Clark et al. (2021-06-23), Clinical stage drugs targeting inhibitor of apoptosis proteins purge episomal Hepatitis B viral genome in preclinical models Cell Death Disease, 2021-06-23;12(7):641.
PMID: 34162831 -
Species: Chicken, Duck
Sample Types: Whole Cells
Applications: BioassayZWM Tong et al. (2021-06-10), Primary Chicken and Duck Endothelial Cells Display a Differential Response to Infection with Highly Pathogenic Avian Influenza Virus Genes, 2021-012(6).
PMID: 34200798 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayJ Kulsuptrak et al. (2021-03-16), A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection Cell Reports, 2021-034(11):108859.
PMID: 33730579 -
Species: Mouse
Sample Types: Organoids
Applications: BioassayP Francica et al. (2020-08-25), Functional Radiogenetic Profiling Implicates ERCC6L2 in Non-homologous End Joining Cell Rep, 2020-032(8):108068.
PMID: 32846126 -
Species: Canine, Human
Sample Types: Whole Cells
Applications: BioassayKD Hulme et al. (2020-07-22), High glucose levels increase influenza-associated damage to the pulmonary epithelial-endothelial barrier Elife, 2020-09(0).
PMID: 32697191 -
Species: Human
Sample Types: Organoid
X Jin et al. (2020-05-11), LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer Mol. Cancer, 2020-019(1):87.
PMID: 32393270 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayX Li et al. (2020-03-18), Breast cancer organoids from a patient with giant papillary carcinoma as a high-fidelity model Cancer Cell Int., 2020-020(0):86.
PMID: 32206037 -
Species: Mouse
Sample Types: Organoids
M Barazas et al. (2018-12-10), Radiosensitivity Is an Acquired Vulnerability of PARPi-Resistant BRCA1-Deficient Tumors Cancer Res., 2018-179(3):452-460.
PMID: 30530501 -
Klueh U et al. (2015-08-25), Basement Membrane-Based Glucose Sensor Coatings Enhance Continuous Glucose Monitoring in Vivo. J Diabetes Sci Technol, 2015-09(5):957-65.
PMID: 26306494 -
Okere B et al. (2015-07-27), In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids. Int J Immunopathol Pharmacol, 2015-028(3):390-402.
PMID: 26216908
There are no citations that match your criteria.
Customer Reviews for Cultrex Basement Membrane Extract, Type 2, Pathclear (1)
Customer Images
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Verified Customer | Posted 08/15/2023Colon organoids were grown in Cultrex. We tested Cultrex when we have a back order of Matrigel and the organoids grow similarly as they are in Matrigel.
There are no reviews that match your criteria.
FAQs for Cultrex Basement Membrane Extract, Type 2, Pathclear
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What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?
Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.
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How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
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How should Cultrex Basement Membrane Extract (BME) be stored and handled?
Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.
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What type of culture plates are recommended for organoid culture and other cultures using Cultrex BME products?
Any plate that is tissue culture treated will bind Cultrex domes/Cultrex coatings. Non-treated or low attachment plates are not recommended for organoid growth and for other cell cultures using coated BME. The domes will float away if non-treated or low-attachment plates are used. Bio-Techne has used the folllowing plates: Corning 96-well, Catalog # 3595; Corning 24-well, Catalog #3526; ThermoFisher 6-well, Catalog # 140675.