Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

FAQs for Cultrex Basement Membrane Extract, Type 2, Pathclear, with Phenol Red

• What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

  Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex^® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

• How should Cultrex Basement Membrane Extract (BME) be stored and handled?

  Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

• What type of culture plates are recommended for organoid culture and other cultures using Cultrex BME products?

  Any plate that is tissue culture treated will bind Cultrex domes/Cultrex coatings. Non-treated or low attachment plates are not recommended for organoid growth and for other cell cultures using coated BME.  The domes will float away if non-treated or low-attachment plates are used. Bio-Techne has used the folllowing plates: Corning 96-well, Catalog # 3595;  Corning 24-well, Catalog #3526;  ThermoFisher 6-well,  Catalog # 140675.