Proteome Profiler Human Chemokine Array Kit

A membrane-based antibody array for the parallel determination of the relative levels of selected human chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, urine and saliva.

Principle of the Array

The Proteome Profiler Human Chemokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Chemokines?

Determining the expression of multiple chemokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

• 4 Array Membranes
• 4-Well Multi-dish
• Array Buffers
• Wash Buffer
• Detection Antibody Cocktail
• Streptavidin-HRP
• Chemiluminescent Detection Reagents
• Transparency Overlay Template
• Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

Scientific Data Examples for Proteome Profiler Human Chemokine Array Kit

Detection of Multiple Analytes in Cell Culture Supernate with the Human Chemokine Array. Cells were untreated or treated as indicated. 500 µL of cell culture supernate was run on each array. Data shown are from a 2 minute exposure to X-ray film. MG-63 human osteosarcoma cells were untreated or treated with 1 ng/mL recombinant human IL-1 beta (R&D Systems, Catalog # 201-LB) for 72 hours.

Detection of Multiple Analytes in Cell Culture Supernate with the Human Chemokine Array. Cells were untreated or treated as indicated. 500 µL of cell culture supernate was run on each array. Data shown are from a 2 minute exposure to X-ray film. THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) for 8 hours, followed by the addition of 1 µg/mL LPS for 16 hours.