Zebrafish Tie-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF928
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Zebrafish
Applications
Immunohistochemistry, Western Blot, Blockade of Receptor-ligand Interaction
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant zebrafish Tie-2
Val22-His741
Accession # O73791
Val22-His741
Accession # O73791
Specificity
Detects zebrafish Tie‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) Tie-2, recombinant mouse Tie-2, and rhTie-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Zebrafish Tie-2 Antibody
Tie-2 in Zebrafish Embryo.
Tie-2 was detected in immersion fixed whole mount zebrafish embryo (24 hours old) using 10 µg/mL Goat Anti-Zebrafish Tie-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF928) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Zebrafish Tie-2 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 4-10 µg/mL of this antibody will block 50% of the binding of 25 ng/mL of biotinylated Recombinant Human Ang-2 to immobilized Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928-T2) coated at 4 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed whole mount zebrafish embryo (24 hours old)
Sample: Immersion fixed whole mount zebrafish embryo (24 hours old)
Western Blot
0.1 µg/mL
Sample: Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928-T2)
Sample: Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928-T2)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tie-2
Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Zebrafish Tie-2 cDNA encodes a 1116 amino acid (aa) residue precursor protein shares 38% sequence homology with human Tie-2 in the extracellular domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind Tie-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects.
References
- Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
- Takakura, N. et al. (1998) Immunity 9:677.
- Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Alternate Names
CD202b, TEK, Tie2
Entrez Gene IDs
Gene Symbol
TEK
UniProt
Additional Tie-2 Products
Product Documents for Zebrafish Tie-2 Antibody
Product Specific Notices for Zebrafish Tie-2 Antibody
For research use only
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