Zebrafish BMP-2/BMP-4 Alexa Fluor® 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1128V
Conjugate
Catalog #
Key Product Details
Species Reactivity
Zebrafish
Applications
Western Blot
Label
Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 169125
Product Specifications
Immunogen
E. coli-derived recombinant zebrafish BMP-4
Ser288-Arg400
Accession # NP_571417
Ser288-Arg400
Accession # NP_571417
Specificity
Detects zebrafish BMP‑2 and BMP‑4 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Zebrafish BMP-2/BMP-4 Alexa Fluor® 405-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: BMP-4
BMP-4 is one of at least 15 structurally and functionally-related BMPs, which are members of the transforming growth factor-beta (TGF-beta) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to have roles in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta superfamily proteins, BMPs are highly conserved across animal species. Zebrafish BMP-4 shares 73% amino acid (aa) sequence identity with human and murine BMP-4, and 86% aa identity with zebrafish BMP-2b. Zebrafish BMP-4 mRNA is detected throughout embryonic development and has been detected in tissues of adult fish, including the brain, heart, digestive tracts, testes, and jaw. BMP-4 has been shown to play roles in directing zebrafish heart looping and in ear development. The combined expression pattern of zBMP-2a/2b/4 coincides with areas where BMP-2/4 expression would be found in other vertebrates. Biologically active BMP-4 is a disulfide-linked homodimer of the carboxy-terminal 113 aa that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond.
Long Name
Bone Morphogenetic Protein 4
Alternate Names
BMP2B, BMP2B1, BMP4, MCOPS6, OFC11
Gene Symbol
BMP4
UniProt
Additional BMP-4 Products
Product Specific Notices for Zebrafish BMP-2/BMP-4 Alexa Fluor® 405-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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