ZEB1 Antibody (CL0151) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52866
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0013.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]")
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0014.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0151
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P37275, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for ZEB1 Antibody (CL0151) - BSA Free
![Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0008.jpg "Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866]")
Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866]
Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis of extracts from RH-30 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-ZEB1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensi![Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0002.jpg "Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866]")
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866]
Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in RH30 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.![Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0007.jpg "Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866]")
![Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0001.jpg "Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866]")
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0009.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]")
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0010.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]")
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]](https://resources.bio-techne.com/images/products/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0011.jpg "Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866]")
Applications for ZEB1 Antibody (CL0151) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZEB1
Long Name
Zinc Finger E-box Binding Homeobox 1
Alternate Names
AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP
Gene Symbol
ZEB1
Additional ZEB1 Products
Product Documents for ZEB1 Antibody (CL0151) - BSA Free
Product Specific Notices for ZEB1 Antibody (CL0151) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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