WT1 Antibody (CL13170) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44378

Novus Biologicals, part of Bio-Techne
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NBP3-44378
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BSA Free
NBP3-18547
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13170

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all WT1 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P19544, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse WT1 Antibody (CL13170) - Azide and BSA Free (NBP3-18547) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for WT1 Antibody (CL13170) - Azide and BSA Free

WT1 Antibody (CL13170) - Azide and BSA Free Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]

Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]

Analysis in human fallopian tube and lymph node tissues using NBP3-44378 antibody. Corresponding WT1 RNA-seq data are presented for the same tissues.
WT1 Antibody (CL13170) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: WT1 Antibody [NBP3-44378]

Immunocytochemistry/ Immunofluorescence: WT1 Antibody [NBP3-44378]

Staining of CACO-2 cells using the Anti-WT1 monoclonal antibody) .
WT1 Antibody (CL13170) - Azide and BSA Free Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]

Immunohistochemistry-Paraffin: WT1 Antibody [NBP3-44378]

Staining of human kidney shows strong nuclear positivity in cells in glomeruli.
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Applications for WT1 Antibody (CL13170) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WT1

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation.

Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Long Name

Wilms Tumor 1

Alternate Names

GUD, WAGR, WIT-2, WT33

Gene Symbol

WT1

Additional WT1 Products

Product Documents for WT1 Antibody (CL13170) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WT1 Antibody (CL13170) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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