Wnt-3a Antibody (3A6) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19050
![Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]](https://resources.bio-techne.com/images/products/Wnt-3a-Antibody-3A6-Western-Blot-NBP1-19050-img0007.jpg "Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3A6
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human Wnt-3a (19-352aa) purified from E. coli (NP_149122).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Wnt-3a Antibody (3A6) - BSA Free
Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]
Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Wnt-3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1: 293T cell lysateLane 2: Wnt3a Transfected 293T cell lysate![Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) - BSA Free [NBP1-19050]](https://resources.bio-techne.com/images/products/Wnt-3a-Antibody-3A6-Immunohistochemistry-Paraffin-NBP1-19050-img0004.jpg "Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) - BSA Free [NBP1-19050]")
Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) - BSA Free [NBP1-19050]
Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) [NBP1-19050] - Human breast cancer tissue were incubated with anti-human Wn-t3a for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).![Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]](https://resources.bio-techne.com/images/products/Wnt-3a-Antibody-3A6-Western-Blot-NBP1-19050-img0006.jpg "Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]")
Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]
Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Applications for Wnt-3a Antibody (3A6) - BSA Free
Application
Recommended Usage
ELISA
1:1000-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:50-1:100SDS-PAGE 1:100-1:2000
Western Blot
1:1000-1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 26708597)
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Wnt-3a
Long Name
Wingless-type MMTV Integration Site Family, Member 3a
Alternate Names
Wnt3a
Entrez Gene IDs
89780 (Human)
Gene Symbol
WNT3A
UniProt
Additional Wnt-3a Products
Product Documents for Wnt-3a Antibody (3A6) - BSA Free
Product Specific Notices for Wnt-3a Antibody (3A6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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