WDR45 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18255
![Western Blot: WDR45 Antibody [NBP3-18255]](https://resources.bio-techne.com/images/products/WDR45 Antibody-Western Blot-NBP3-18255-img0002.jpg "Western Blot: WDR45 Antibody [NBP3-18255]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide from the C-terminal of Human WDR45(aa. 312-322)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit WDR45 Antibody - BSA Free (NBP3-18255) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for WDR45 Antibody - BSA Free
Western Blot: WDR45 Antibody [NBP3-18255]
Western Blot: WDR45 Antibody [NBP3-18255] - Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 40 kDa WDR45 protein using Rabbit Anti-WDR45 Polyclonal Antibody (NBP3-18255). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody (NBP3-18255) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 40 kDa.![Immunocytochemistry/ Immunofluorescence: WDR45 Antibody [NBP3-18255]](https://resources.bio-techne.com/images/products/WDR45-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18255-img0001.jpg "Immunocytochemistry/ Immunofluorescence: WDR45 Antibody [NBP3-18255]")
Immunocytochemistry/ Immunofluorescence: WDR45 Antibody [NBP3-18255]
Immunocytochemistry/Immunofluorescence: WDR45 Antibody [NBP3-18255] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDR45 Polyclonal Antibody (NBP3-18255). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody (NBP3-18255) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WDR45 Antibody (D) Composite.Applications for WDR45 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS, 50% glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: WDR45
Alternate Names
JM5, WD repeat domain 45, WD repeat domain phosphoinositide-interacting protein 4, WD repeat-containing protein 45, WD45 repeat protein interacting with phosphoinositides 4, WDRX1, WDRXI4, WIPI-4, X-linked 1
Gene Symbol
WDR45
Additional WDR45 Products
Product Documents for WDR45 Antibody - BSA Free
Product Specific Notices for WDR45 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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