VISTA/B7-H5/PD-1H Antibody (MH5A) [Alexa Fluor® 700]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11969AF700
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Block/Neutralize, ELISA, Immunohistochemistry
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Monoclonal Armenian Hamster IgG Lambda Clone # MH5A
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The antibody was generated by immunising Armenian hamsters with mVISTA/B7-H5/PD-1HIg.
Specificity
This antibody specifically binds VISTA/B7-H5/PD-1H.
Clonality
Monoclonal
Host
Armenian Hamster
Isotype
IgG Lambda
Applications for VISTA/B7-H5/PD-1H Antibody (MH5A) [Alexa Fluor® 700]
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: VISTA/B7-H5/PD-1H
VISTA (V-domain immunoglobulin suppressor of T cell activation) is a cell surface coinhibitory protein belonging to the CD28/B7 family. VISTA can act as both a ligand and a receptor in regulating T cell activity. VISTA is expressed on dendritic cells, monocytes, and both CD4+ and CD8+ T cells. VISTA functions as an inhibitory receptor downregulating CD4+ and CD8+ T cell proliferation and exhibiting a protective effect in mice from graft versus host disease, asthma, and acute hepatitis. VISTA also acts as a ligand on antigen presenting cells. Two cell surface expressed proteins VSIG-3 and PSGL-1 have been identified as binding partners of VISTA. Immune checkpoint proteins like the VISTA protein are often overexpressed by cancer cells or surrounding cells in the tumor microenvironment. VISTA upregulation occurs on tumor-infiltrating leukocytes and is expressed on several cancer types including melanoma, lung, ovarian, colon, prostate, and pancreatic cancer. Further, knockout and blocking antibody studies of VISTA have shown an increase in T cell responses in several mouse tumor models making VISTA a potential therapeutic target alone or in combination with other known immune checkpoint inhibitors. Alternative names of VISTA include PD-1H, Gi24, Dies1, and SISP1.
Alternate Names
4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR
Gene Symbol
VSIR
Additional VISTA/B7-H5/PD-1H Products
Product Documents for VISTA/B7-H5/PD-1H Antibody (MH5A) [Alexa Fluor® 700]
Product Specific Notices for VISTA/B7-H5/PD-1H Antibody (MH5A) [Alexa Fluor® 700]
Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...