UBE2I/Ubc9 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00096
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human UBE2I/Ubc9 (Accession#: P63279; Met1-Ser158)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for UBE2I/Ubc9 Antibody - Azide and BSA Free
Immunoprecipitation: UBE2I/Ubc9 Antibody [NBP3-00096] -
Immunoprecipitation: UBE2I/Ubc9 Antibody [NBP3-00096] - UBE2I was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate Lane C:0.5 mg A431 Whole Cell Lysate 2 uL NBP3-00096 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00096, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 18 kDa Observed band size: 18 kDa
Western Blot: UBE2I/Ubc9 Antibody [NBP3-00096] -
Western Blot: UBE2I/Ubc9 Antibody [NBP3-00096] - NBP3-00096 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: NIH-3T3 Whole Cell Lysate Lane D: PC12 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:18 kDa
Immunocytochemistry/ Immunofluorescence: UBE2I/Ubc9 Antibody [NBP3-00096] -
Immunocytochemistry/ Immunofluorescence: UBE2I/Ubc9 Antibody [NBP3-00096] - Immunofluorescence staining of UBE2I/Ubc9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-00096 (dilution ratio 1:1000) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.Applications for UBE2I/Ubc9 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:300-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UBE2I/Ubc9
Long Name
Ubiquitin-conjugating Enzyme E2I
Alternate Names
p18, SUMO-protein Ligase, Ubc9, UBCE9
Gene Symbol
UBE2I
Additional UBE2I/Ubc9 Products
Product Documents for UBE2I/Ubc9 Antibody - Azide and BSA Free
Product Specific Notices for UBE2I/Ubc9 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...