Tryptase beta-2/TPSB2 Antibody (9H6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26273

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9H6

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Tryptase beta-2/TPSB2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Tryptase beta-2/TPSB2 [UniProt P20231]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Tryptase beta-2/TPSB2 Antibody (9H6) - BSA Free

Tryptase beta-2/TPSB2 Antibody (9H6)

Western Blot: Tryptase beta-2/TPSB2 Antibody (9H6) [NBP3-26273] -

Western Blot: Tryptase beta-2/TPSB2 Antibody (9H6) [NBP3-26273] - Positive Western Blot detected in: Mouse Kidney tissue lysate.
All lanes: Tryptase beta-2/TPSB2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Tryptase beta-2/TPSB2 Antibody (9H6)

Flow Cytometry: Tryptase beta-2/TPSB2 Antibody (9H6) [NBP3-26273] -

Flow Cytometry: Tryptase beta-2/TPSB2 Antibody (9H6) [NBP3-26273] - Overlay Peak curve showing K562 cells stained with Tryptase beta-2/TPSB2 Antibody (9H6) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Tryptase beta-2/TPSB2 Antibody (9H6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Tryptase beta-2/TPSB2

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq]

Alternate Names

EC 3.4.21, EC 3.4.21.59, mast cell tryptase beta II, mast cell tryptase beta III, TPS2, TPS2tryptase beta-2, TPSB2, tryptase beta 2, tryptase beta 2 (gene/pseudogene), tryptase beta II, tryptase beta III, Tryptase beta2, Tryptase beta-2, Tryptase II, tryptase III, Tryptase-2, tryptaseB, tryptaseC, Tryptase-C

Gene Symbol

TPSB2

Additional Tryptase beta-2/TPSB2 Products

Product Documents for Tryptase beta-2/TPSB2 Antibody (9H6) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tryptase beta-2/TPSB2 Antibody (9H6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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