Tryptase alpha/beta 1 Antibody (AA1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26444

Novus Biologicals, part of Bio-Techne
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NBP2-26444
NBP2-26444-0.05ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # AA1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Tryptase alpha/beta 1 Antibodies »

Product Specifications

Immunogen

BALB/C mice were injected with a purified human lung tryptase.

Reactivity Notes

Rat and mouse reactivity reported in scientific literature. Please see Publications tab for full list. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Specificity

Due to sequence identity this antibody will likely cross react with tryptase beta 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Tryptase alpha/beta 1 Antibody (AA1)

Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (AA1) [NBP2-26444]

Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (AA1) [NBP2-26444]

Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP2-26444] - Human tonsil stained with Mast Cell Tryptase antibody.
Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (AA1) [NBP2-26444]

Immunohistochemistry-Paraffin: Tryptase alpha/beta 1 Antibody (AA1) [NBP2-26444]

Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP2-26444] - Analysis of Mast Cell Tryptase formalin-fixed paraffin embedded human tonsil using NBP2-26444.
Tryptase alpha/beta 1 Antibody (AA1)

Immunohistochemistry-Paraffin: Mouse Monoclonal Tryptase alpha/beta 1 Antibody (AA1) [IMGENEX: IMG-80250] [NBP2-26444]

Immunohistochemistry-Paraffin: Mouse Monoclonal Tryptase alpha/beta 1 Antibody (AA1) [IMGENEX: IMG-80250] [NBP2-26444] - Analysis of Tryptase alpha/beta 1 in human liver tissue. Mast cell positive for Tryptase alpha/beta 1. pAb: x500 and DAPI counter stain. Image from a verified customer review.

Applications for Tryptase alpha/beta 1 Antibody (AA1)

Application
Recommended Usage

Immunohistochemistry

1:300-1:600

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:300-1:600

Western Blot

1:100-1:2000
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Reviewed Applications

Read 1 review rated 5 using NBP2-26444 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Tryptase alpha/beta 1

Principal Names: Tryptase alpha/beta 1 Official Gene Symbol: TPSAB1 Gene ID: 7177 (Human) Gene Map Locus: 16p13.3 (Human). Human tryptases are divided into two groups, alpha and beta. beta-Tryptase appears to be the major type stored in mast cell secretory granules and alpha-tryptase is the major isoform expressed by basophils.beta-tryptase, a major constituent of mast cell secretory granules, has emerged recently as the most specific marker in vivo of mast cell activation in humans. It is a trypsin like serine protease that hydrolyzes peptide bonds on the C-terminal side of basic amino acids. Unlike trypsin, however, it is highly selective in hydrolyzing its peptide and protein targets and resists inactivation by circulating inhibitors. It has potential roles in the lung, skin, and other tissues as a local anticoagulant, activator of collagenase, promoter of smooth muscle contraction, and modulator of neuropeptide activity. The pathogenic role for tryptases has been observed in asthmatic airway inflammation other inflammatory disorders. The gene is mapped on human chromosome 16p13.3.

Alternate Names

alpha-Tryptase, EC:3.4.21.59, epididymis secretory sperm binding protein, mast cell alpha II tryptase, mast cell beta I tryptase, mast cell tryptase, TPS1, TPS2, TPSAB1, TPSB1, TPSB2, Tryptase 1, tryptase alpha II, Tryptase alpha-1, tryptase beta I, tryptase beta-1, Tryptase beta-2, Tryptase I, tryptase II, tryptase-1, tryptase-2, tryptase-I, tryptase-III

Gene Symbol

TPSAB1

Additional Tryptase alpha/beta 1 Products

Product Documents for Tryptase alpha/beta 1 Antibody (AA1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tryptase alpha/beta 1 Antibody (AA1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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