TLR9 Antibody (26C593.2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24863
![Flow (Intracellular): TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]](https://resources.bio-techne.com/images/products/TLR9-Antibody-26C593-2-Azide-Free-Flow-Intracellular-NBP2-24863-img0008.jpg "Flow (Intracellular): TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]")
![Western Blot: TLR9 Antibody (26C593.2)Azide Free [NBP2-24863]](https://resources.bio-techne.com/images/products/TLR9-Antibody-26C593-2-Azide-Free-Western-Blot-NBP2-24863-img0006.jpg "Western Blot: TLR9 Antibody (26C593.2)Azide Free [NBP2-24863]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Equine, Monkey, Primate
Cited:
Human, Primate (Rhesus monkey)
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Functional, In vitro assay, Knockdown Validated, CyTOF-ready
Cited:
Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry Control, In vitro assay, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 26C593.2
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).
Reactivity Notes
Rhesus Monkey.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for TLR9 Antibody (26C593.2) - Azide and BSA Free
Western Blot: TLR9 Antibody (26C593.2)Azide Free [NBP2-24863]
Western Blot: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 ug/ml.![Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]](https://resources.bio-techne.com/images/products/TLR9-Antibody-26C593-2-Azide-Free-Immunohistochemistry-Paraffin-NBP2-24863-img0007.jpg "Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]")
Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]
Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Formalin-fixed, paraffin-embedded human spleen probed with TLR9 antibody at 5 ug/ml. Human tissue was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.![Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]](https://resources.bio-techne.com/images/products/TLR9-Antibody-26C593-2-Azide-Free-Flow-Cytometry-NBP2-24863-img0003.jpg "Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]")
Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]
Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Intracellular flow analysis of TLR9 in Ramos cells using 0.5 ug of this antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents anti-TLR9 antibody.![Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]](https://resources.bio-techne.com/images/products/TLR9-Antibody-26C593-2-Azide-Free-Flow-Cytometry-NBP2-24863-img0009.jpg "Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]")
![Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]](https://resources.bio-techne.com/images/products/TLR9-Antibody-26C593-2-Azide-Free-Flow-Cytometry-NBP2-24863-img0010.jpg "Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]")
![Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]](https://resources.bio-techne.com/images/products/TLR9-Antibody-26C593-2-Azide-Free-Flow-Cytometry-NBP2-24863-img0011.jpg "Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]")
![Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]](https://resources.bio-techne.com/images/products/TLR9-Antibody-26C593-2-Azide-Free-Flow-Cytometry-NBP2-24863-img0012.jpg "Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]")
![Simple Western: TLR9 Antibody (26C593.2)Azide Free [NBP2-24863]](https://resources.bio-techne.com/images/products/TLR9-Antibody-26C593-2-Azide-Free-Simple-Western-NBP2-24863-img0014.jpg "Simple Western: TLR9 Antibody (26C593.2)Azide Free [NBP2-24863]")
Applications for TLR9 Antibody (26C593.2) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow (Intracellular)
1:10 - 1:1000
Flow Cytometry
1ul/1 million cells
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry-Paraffin
5 ug/ml
Immunoprecipitation
1:10 - 1:500
Simple Western
30 ug/ml
Western Blot
2-5 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 17116765). Use in Immunohistochemistry reported in scientific literature (PMID: 16477017). Use in FLOW cell surface reported in scientific literature (PMID: 15879022).
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Sterile - filtered PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TLR9
Long Name
Toll-like Receptor 9
Alternate Names
CD289
Gene Symbol
TLR9
Additional TLR9 Products
Product Documents for TLR9 Antibody (26C593.2) - Azide and BSA Free
Product Specific Notices for TLR9 Antibody (26C593.2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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