TLE1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-04105
![Immunohistochemistry-Paraffin: TLE1 Antibody [NBP2-04105]](https://resources.bio-techne.com/images/products/TLE1-Antibody-Immunohistochemistry-NBP2-04105-img0002.jpg "Immunohistochemistry-Paraffin: TLE1 Antibody [NBP2-04105]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human Transducin-like Enhancer of Split 1 using the numbering given in entry NP_005068.2 (GeneID 7088).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit TLE1 Antibody - BSA Free (NBP2-04105) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TLE1 Antibody - BSA Free
Immunohistochemistry-Paraffin: TLE1 Antibody [NBP2-04105]
Immunohistochemistry-Paraffin: TLE1 Antibody [NBP2-04105] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TLE1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).![Immunoprecipitation: TLE1 Antibody [NBP2-04105]](https://resources.bio-techne.com/images/products/TLE1-Antibody-Immunoprecipitation-NBP2-04105-img0001.jpg "Immunoprecipitation: TLE1 Antibody [NBP2-04105]")
Immunoprecipitation: TLE1 Antibody [NBP2-04105]
Immunoprecipitation: TLE1 Antibody [NBP2-04105] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TLE1 antibody NBP2-04105 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. TLE1 was also immunoprecipitated by rabbit anti-TLE1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for TLE1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TLE1
Long Name
Transducin-like Enhancer of Split 1
Alternate Names
GRG1
Gene Symbol
TLE1
UniProt
Additional TLE1 Products
Product Documents for TLE1 Antibody - BSA Free
Product Specific Notices for TLE1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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