Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25719
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1140 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Thrombospondin-2. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free (NBP3-25719) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free
Western Blot: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] -
Western Blot: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - AsPC-1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] -
Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:500. Blue: Fluoroshield with DAPI .
ELISA: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] -
ELISA: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Sandwich ELISA detection of recombinant human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1194] (NBP3-25720) (5 ug/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1140] (NBP3-25719) (1 ug/mL) Please notice that NBP3-25719 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25720. Please contact us for custom HRP-conjugated antibody.Applications for Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free
Application
Recommended Usage
ELISA
Assay dependent
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Sandwich ELISA
Assay dependent
Western Blot
Assay dependent
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Thrombospondin-2
Alternate Names
THBS2, Thrombospondin2, TSP-2
Gene Symbol
THBS2
Additional Thrombospondin-2 Products
Product Documents for Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free
Product Specific Notices for Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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