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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

PerCP (Excitation = 490 nm, Emission = 675 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 409522

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human TGR5/GPBAR1
Met1-Asn330
Accession # Q8TDU6

Specificity

Stains human TGR5/GPBAR1 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for TGR5/GPBAR1 Antibody (409522) [PerCP]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB4286. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TGR5/GPBAR1

TGR5, also called GPBAR1, M-BAR, or BG37 or is a G-protein coupled receptor superfamily member that is a widely expressed cell surface receptor for bile acids. On macrophages, bile acid engagement of TGR5/GPBAR1 suppresses phagocytosis and other functions. Extracellular portions of human TGR5/GPBAR1 show approximately 75% amino acid identity with corresponding regions of mouse TGR5/GPBAR1.

Long Name

Bile Acid Receptor TGR5

Alternate Names

BG37, GPBA, GPBAR1, GPCR19, GPR131, M-BAR

Gene Symbol

GPBAR1

Additional TGR5/GPBAR1 Products

Product Documents for TGR5/GPBAR1 Antibody (409522) [PerCP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TGR5/GPBAR1 Antibody (409522) [PerCP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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