TFEB Antibody (CL14112) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44287

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL14112

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

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Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P19484, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Novus Biologicals Mouse TFEB Antibody (CL14112) - Azide and BSA Free (NBP3-26853) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TFEB Antibody (CL14112) - Azide and BSA Free

TFEB Antibody (CL14112) - Azide and BSA Free Immunohistochemistry-Paraffin: TFEB Antibody [NBP3-44287]

Immunohistochemistry-Paraffin: TFEB Antibody [NBP3-44287]

Analysis in human tonsil and liver tissues using NBP3-44287 antibody. Corresponding TFEB RNA-seq data are presented for the same tissues.
TFEB Antibody (CL14112) - Azide and BSA Free Immunohistochemistry-Paraffin: TFEB Antibody [NBP3-44287]

Immunohistochemistry-Paraffin: TFEB Antibody [NBP3-44287]

Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.
TFEB Antibody (CL14112) - Azide and BSA Free Immunohistochemistry-Paraffin: TFEB Antibody [NBP3-44287]

Immunohistochemistry-Paraffin: TFEB Antibody [NBP3-44287]

Staining of human liver shows very weak positivity in hepatocytes.
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Applications for TFEB Antibody (CL14112) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFEB

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Long Name

Transcription Factor EB

Alternate Names

ALPHATFEB, BHLHE35, TCFEB

Gene Symbol

TFEB

Additional TFEB Products

Product Documents for TFEB Antibody (CL14112) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TFEB Antibody (CL14112) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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