TCF-2/HNF-1 beta Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89680

Novus Biologicals, part of Bio-Techne
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NBP1-89680
NBP1-89680-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunocytochemistry/ Immunofluorescence, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TCF-2/HNF-1 beta Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQH

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32008054).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit TCF-2/HNF-1 beta Antibody - BSA Free (NBP1-89680) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, Simple Western and ChIP. Anti-TCF-2/HNF-1 beta Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TCF-2/HNF-1 beta Antibody - BSA Free

TCF-2/HNF-1 beta Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: TCF-2/HNF-1 beta Antibody - BSA Free [NBP1-89680]

Immunocytochemistry/ Immunofluorescence: TCF-2/HNF-1 beta Antibody - BSA Free [NBP1-89680]

Staining of human cell line CACO-2 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Nuclear expression of HNF1B (green) in a subset of PDX1 expressing cells (blue) in human pancreas. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP1-89680] - Staining of human kidney shows moderate to strong nuclear positivity in renal tubules.
View 7 more images

Applications for TCF-2/HNF-1 beta Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Reported in scientific literature (PMID:32008054)

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Simple Western

1:30

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in A549, separated by Size, antibody dilution of 1:30, apparent MW was 66 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 5 using NBP1-89680 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF-2/HNF-1 beta

TCF2 encodes transcription factor 2, a liver-specific factor of the homeobox-containing basic helix-turn-helix family. The TCF2 protein is believed to form heterodimers with another liver-specific member of this transcription factor family, TCF1; depending on the TCF2 isoform, the result may be to activate or inhibit transcription of target genes. Mutation of TCF2 that disrupts normal function has been identified as the cause of MODY5 (Maturity-Onset of Diabetes, Type 5). A third human transcript variant is believed to exist based on such a variant in the rat: however, to date such an mRNA species has not been isolated.

Long Name

Transcription Factor 2

Alternate Names

HNF-1 beta, HNF1B, HNF2, LF-B3, MODY5, TCF2, VHNF1

Entrez Gene IDs

6928 (Human)

Gene Symbol

HNF1B

UniProt

P35680

Additional TCF-2/HNF-1 beta Products

Product Documents for TCF-2/HNF-1 beta Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TCF-2/HNF-1 beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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